Pulmonary surfactant-associated protein A as a marker of respiratory distress in forensic pathology: Assessment of the immunohistochemical and biochemical findings

Hitoshi Maeda, Masaki Q Fujita, Bao Li Zhu, Kaori Ishida, Li Quan, Shigeki Oritani, Mari Taniguchi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The aim of the present study was to review the immunohistochemical and biochemical findings with reference to the causes of death in routine casework (total, n = 492). In the immunohistochemistry (n = 283), an increase in intra-alveolar granular SP-A (SP-A score) was often observed in asphyxiation (n = 21/34, 61.8%) and freshwater drowning (n = 15/24, 62.5%), and also in fire and methamphetamine (MA) fatalities (n = 22/76, 28.9% and n = 5/16, 31.3%). Serum SP-A level (n = 134) was elevated in acute respiratory distress syndrome and in some cases of drowning, fire and MA fatalities, hyperthermia and chest traumas. A quantitative analysis of SP-A subclass-gene expression (SP-A1/A2 mRNA) in the lung tissue specimens (n = 126) revealed an increase in the SP-A1/A2 mRNA ratio in asphyxiation (n = 17/21, 80.9%), freshwater drowning (n = 7/9, 77.7%), fire and MA fatalities (n = 20/35, 57.1% and n = 8/10, 80.0%). These findings suggested the usefulness of SP-A as a marker of asphyxiation, respiratory distress and alveolar injury.

Original languageEnglish
JournalLegal Medicine
Volume5
Issue numberSUPPL. 1
DOIs
Publication statusPublished - 2003
Externally publishedYes

Fingerprint

Forensic Pathology
Pulmonary Surfactant-Associated Protein A
Methamphetamine
Asphyxia
Fresh Water
varespladib methyl
Messenger RNA
Adult Respiratory Distress Syndrome
Wounds and Injuries
Cause of Death
Fever
Thorax
Immunohistochemistry
Gene Expression
Lung
Serum

Keywords

  • Asphyxiation
  • Blood biochemistry
  • Forensic pathology
  • Immunohistochemistry
  • Pulmonary surfactant-associated protein A
  • Respiratory distress
  • RNA assay

ASJC Scopus subject areas

  • Genetics
  • Pathology and Forensic Medicine
  • Psychiatry and Mental health
  • Public Health, Environmental and Occupational Health

Cite this

Pulmonary surfactant-associated protein A as a marker of respiratory distress in forensic pathology : Assessment of the immunohistochemical and biochemical findings. / Maeda, Hitoshi; Fujita, Masaki Q; Zhu, Bao Li; Ishida, Kaori; Quan, Li; Oritani, Shigeki; Taniguchi, Mari.

In: Legal Medicine, Vol. 5, No. SUPPL. 1, 2003.

Research output: Contribution to journalArticle

Maeda, Hitoshi ; Fujita, Masaki Q ; Zhu, Bao Li ; Ishida, Kaori ; Quan, Li ; Oritani, Shigeki ; Taniguchi, Mari. / Pulmonary surfactant-associated protein A as a marker of respiratory distress in forensic pathology : Assessment of the immunohistochemical and biochemical findings. In: Legal Medicine. 2003 ; Vol. 5, No. SUPPL. 1.
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