Pulmonary surfactant-associated protein A levels in cadaveric sera with reference to the cause of death

K. Ishida, B. L. Zhu, Li Quan, Masaki Q Fujita, H. Maeda

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Pulmonary surfactant-associated protein A (SP-A) is an exclusively lung specific protein, and is considered to leak into the blood stream in alveolar septal damage. In this study we examined the serum SP-A level in forensic autopsy materials using an enzyme immunoassay with monoclonal antibodies to assess the postmortem level in relation to the cause and mode of death. Although a gradual postmortem degradation should be taken into consideration, topological relationship of serum level seemed to be fairly stable (arterial≥venous blood in most cases), indicating no evident influence of postmortem diffusion. Significant elevation of serum SP-A (76.7-250 ng/ml in left heart blood) was observed in hyaline membrane diseases from various causes independent of the postmortem intervals (<30 h). However, mean SP-A levels in postmortem heart blood were usually low in asphyxia including hanging, strangulation and choking (left, 25.5 ng/ml; right, 22.3 ng/ml), polytrauma (left, 13.1 ng/ml; right, 9.0 ng/ml) and stab wound to the neck (left, 34.1 ng/ml; right, 29.4 ng/ml). Prominent elevation was noted in a case of fatal strangulation with complication of idiopathic interstitial pneumonia, and also in some deaths due to drowning, burns in fires, blunt and gunshot chest injuries. These findings indicated that postmortem elevation of serum SP-A levels was associated with alveolar septal damage due to inflammation, mechanical and physical stresses, which caused leakage of SP-A into the bloodstream. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)125-133
Number of pages9
JournalForensic Science International
Volume109
Issue number2
DOIs
Publication statusPublished - 2000 Mar 27
Externally publishedYes

Fingerprint

Pulmonary Surfactant-Associated Protein A
Staphylococcal Protein A
Surface-Active Agents
Cause of Death
Blood
Serum
Idiopathic Interstitial Pneumonias
Hyaline Membrane Disease
Stab Wounds
Thoracic Injuries
Mechanical Stress
Multiple Trauma
Asphyxia
Airway Obstruction
Immunoenzyme Techniques
Burns
Autopsy
Fires
Neck
Monoclonal Antibodies

Keywords

  • Alveoli-capillary membrane
  • Enzyme immunoassay
  • Forensic pathology
  • Hyaline membrane diseases
  • Surfactant-associated protein A

ASJC Scopus subject areas

  • Genetics
  • Analytical Chemistry
  • Pathology and Forensic Medicine
  • Psychiatry and Mental health

Cite this

Pulmonary surfactant-associated protein A levels in cadaveric sera with reference to the cause of death. / Ishida, K.; Zhu, B. L.; Quan, Li; Fujita, Masaki Q; Maeda, H.

In: Forensic Science International, Vol. 109, No. 2, 27.03.2000, p. 125-133.

Research output: Contribution to journalArticle

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