Pulsatile cardiac tissue grafts using a novel three-dimensional cell sheet manipulation technique functionally integrates with the host heart, in vivo

Akira Furuta, Shunichiro Miyoshi, Yuji Itabashi, Tatsuya Shimizu, Shinichiro Kira, Keiko Hayakawa, Nobuhiro Nishiyama, Kojiro Tanimoto, Yoko Hagiwara, Toshiaki Satoh, Keiichi Fukuda, Teruo Okano, Satoshi Ogawa

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

We devised a method of fabricating easily transplantable scaffoldless 3D heart tissue, made with a novel cell-sheet (CS) technology from cultured cardiomyocytes using a fibrin polymer coated dish. In the present study, we tested in vivo electrical communication which is essential for improving heart function between the host heart and the grafted CS. The epicardial surface of the ventricle of an anesthetized open-chest nude rat was ablated by applying a heated metal. Bilayered CS was obtained from neonatal rat primary culture. CS was transplanted onto the injured myocardial surface (sMI) (sMI+sheet group). The rats were allowed to recover for 1 to 4 weeks, to stabilize the grafts. Action potentials (APs) from the excised perfused heart were monitored by the fluorescence signal of di-4ANEPPS with a high speed charge-coupled device camera. The APs were observed under epicardial pacing of the host heart or the CS grafts. The pacing threshold of the current output was measured in the sMI+sheet group and in the nongrafted sMI group at the center of the sMI and in the normal zone (Nz). Bidirectional AP propagation between the sMI and Nz was observed in the sMI+sheet group (n=14), but was blocked at the marginal area of the sMI in the sMI group (n=9). The ratio of the pacing threshold (sMI/Nz) was significantly lower in the sMI+sheet than in the sMI group (3.0±0.7, 19.0±6.1 respectively P<0.05). There were neither spontaneous nor pacing-induced arrhythmias in these two groups. Bidirectional smooth AP propagation between the host heart and the grafted CS was observed. This finding suggested functional integration of this CS graft with the host heart without serious arrhythmia.

Original languageEnglish
Pages (from-to)705-712
Number of pages8
JournalCirculation Research
Volume98
Issue number5
DOIs
Publication statusPublished - 2006 Mar

Fingerprint

Transplants
Action Potentials
Cardiac Arrhythmias
Nude Rats
Fibrin
Cardiac Myocytes
Polymers
Thorax
Fluorescence
Metals
Communication
Technology
Equipment and Supplies

Keywords

  • Arrhythmia
  • Cardiac transplantation
  • Electrophysiology
  • Regenerative medicine
  • Stem cell-based therapy

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Pulsatile cardiac tissue grafts using a novel three-dimensional cell sheet manipulation technique functionally integrates with the host heart, in vivo. / Furuta, Akira; Miyoshi, Shunichiro; Itabashi, Yuji; Shimizu, Tatsuya; Kira, Shinichiro; Hayakawa, Keiko; Nishiyama, Nobuhiro; Tanimoto, Kojiro; Hagiwara, Yoko; Satoh, Toshiaki; Fukuda, Keiichi; Okano, Teruo; Ogawa, Satoshi.

In: Circulation Research, Vol. 98, No. 5, 03.2006, p. 705-712.

Research output: Contribution to journalArticle

Furuta, A, Miyoshi, S, Itabashi, Y, Shimizu, T, Kira, S, Hayakawa, K, Nishiyama, N, Tanimoto, K, Hagiwara, Y, Satoh, T, Fukuda, K, Okano, T & Ogawa, S 2006, 'Pulsatile cardiac tissue grafts using a novel three-dimensional cell sheet manipulation technique functionally integrates with the host heart, in vivo', Circulation Research, vol. 98, no. 5, pp. 705-712. https://doi.org/10.1161/01.RES.0000209515.59115.70
Furuta, Akira ; Miyoshi, Shunichiro ; Itabashi, Yuji ; Shimizu, Tatsuya ; Kira, Shinichiro ; Hayakawa, Keiko ; Nishiyama, Nobuhiro ; Tanimoto, Kojiro ; Hagiwara, Yoko ; Satoh, Toshiaki ; Fukuda, Keiichi ; Okano, Teruo ; Ogawa, Satoshi. / Pulsatile cardiac tissue grafts using a novel three-dimensional cell sheet manipulation technique functionally integrates with the host heart, in vivo. In: Circulation Research. 2006 ; Vol. 98, No. 5. pp. 705-712.
@article{f62164abad0043f1942c6b9dc26f2696,
title = "Pulsatile cardiac tissue grafts using a novel three-dimensional cell sheet manipulation technique functionally integrates with the host heart, in vivo",
abstract = "We devised a method of fabricating easily transplantable scaffoldless 3D heart tissue, made with a novel cell-sheet (CS) technology from cultured cardiomyocytes using a fibrin polymer coated dish. In the present study, we tested in vivo electrical communication which is essential for improving heart function between the host heart and the grafted CS. The epicardial surface of the ventricle of an anesthetized open-chest nude rat was ablated by applying a heated metal. Bilayered CS was obtained from neonatal rat primary culture. CS was transplanted onto the injured myocardial surface (sMI) (sMI+sheet group). The rats were allowed to recover for 1 to 4 weeks, to stabilize the grafts. Action potentials (APs) from the excised perfused heart were monitored by the fluorescence signal of di-4ANEPPS with a high speed charge-coupled device camera. The APs were observed under epicardial pacing of the host heart or the CS grafts. The pacing threshold of the current output was measured in the sMI+sheet group and in the nongrafted sMI group at the center of the sMI and in the normal zone (Nz). Bidirectional AP propagation between the sMI and Nz was observed in the sMI+sheet group (n=14), but was blocked at the marginal area of the sMI in the sMI group (n=9). The ratio of the pacing threshold (sMI/Nz) was significantly lower in the sMI+sheet than in the sMI group (3.0±0.7, 19.0±6.1 respectively P<0.05). There were neither spontaneous nor pacing-induced arrhythmias in these two groups. Bidirectional smooth AP propagation between the host heart and the grafted CS was observed. This finding suggested functional integration of this CS graft with the host heart without serious arrhythmia.",
keywords = "Arrhythmia, Cardiac transplantation, Electrophysiology, Regenerative medicine, Stem cell-based therapy",
author = "Akira Furuta and Shunichiro Miyoshi and Yuji Itabashi and Tatsuya Shimizu and Shinichiro Kira and Keiko Hayakawa and Nobuhiro Nishiyama and Kojiro Tanimoto and Yoko Hagiwara and Toshiaki Satoh and Keiichi Fukuda and Teruo Okano and Satoshi Ogawa",
year = "2006",
month = "3",
doi = "10.1161/01.RES.0000209515.59115.70",
language = "English",
volume = "98",
pages = "705--712",
journal = "Circulation Research",
issn = "0009-7330",
publisher = "Lippincott Williams and Wilkins",
number = "5",

}

TY - JOUR

T1 - Pulsatile cardiac tissue grafts using a novel three-dimensional cell sheet manipulation technique functionally integrates with the host heart, in vivo

AU - Furuta, Akira

AU - Miyoshi, Shunichiro

AU - Itabashi, Yuji

AU - Shimizu, Tatsuya

AU - Kira, Shinichiro

AU - Hayakawa, Keiko

AU - Nishiyama, Nobuhiro

AU - Tanimoto, Kojiro

AU - Hagiwara, Yoko

AU - Satoh, Toshiaki

AU - Fukuda, Keiichi

AU - Okano, Teruo

AU - Ogawa, Satoshi

PY - 2006/3

Y1 - 2006/3

N2 - We devised a method of fabricating easily transplantable scaffoldless 3D heart tissue, made with a novel cell-sheet (CS) technology from cultured cardiomyocytes using a fibrin polymer coated dish. In the present study, we tested in vivo electrical communication which is essential for improving heart function between the host heart and the grafted CS. The epicardial surface of the ventricle of an anesthetized open-chest nude rat was ablated by applying a heated metal. Bilayered CS was obtained from neonatal rat primary culture. CS was transplanted onto the injured myocardial surface (sMI) (sMI+sheet group). The rats were allowed to recover for 1 to 4 weeks, to stabilize the grafts. Action potentials (APs) from the excised perfused heart were monitored by the fluorescence signal of di-4ANEPPS with a high speed charge-coupled device camera. The APs were observed under epicardial pacing of the host heart or the CS grafts. The pacing threshold of the current output was measured in the sMI+sheet group and in the nongrafted sMI group at the center of the sMI and in the normal zone (Nz). Bidirectional AP propagation between the sMI and Nz was observed in the sMI+sheet group (n=14), but was blocked at the marginal area of the sMI in the sMI group (n=9). The ratio of the pacing threshold (sMI/Nz) was significantly lower in the sMI+sheet than in the sMI group (3.0±0.7, 19.0±6.1 respectively P<0.05). There were neither spontaneous nor pacing-induced arrhythmias in these two groups. Bidirectional smooth AP propagation between the host heart and the grafted CS was observed. This finding suggested functional integration of this CS graft with the host heart without serious arrhythmia.

AB - We devised a method of fabricating easily transplantable scaffoldless 3D heart tissue, made with a novel cell-sheet (CS) technology from cultured cardiomyocytes using a fibrin polymer coated dish. In the present study, we tested in vivo electrical communication which is essential for improving heart function between the host heart and the grafted CS. The epicardial surface of the ventricle of an anesthetized open-chest nude rat was ablated by applying a heated metal. Bilayered CS was obtained from neonatal rat primary culture. CS was transplanted onto the injured myocardial surface (sMI) (sMI+sheet group). The rats were allowed to recover for 1 to 4 weeks, to stabilize the grafts. Action potentials (APs) from the excised perfused heart were monitored by the fluorescence signal of di-4ANEPPS with a high speed charge-coupled device camera. The APs were observed under epicardial pacing of the host heart or the CS grafts. The pacing threshold of the current output was measured in the sMI+sheet group and in the nongrafted sMI group at the center of the sMI and in the normal zone (Nz). Bidirectional AP propagation between the sMI and Nz was observed in the sMI+sheet group (n=14), but was blocked at the marginal area of the sMI in the sMI group (n=9). The ratio of the pacing threshold (sMI/Nz) was significantly lower in the sMI+sheet than in the sMI group (3.0±0.7, 19.0±6.1 respectively P<0.05). There were neither spontaneous nor pacing-induced arrhythmias in these two groups. Bidirectional smooth AP propagation between the host heart and the grafted CS was observed. This finding suggested functional integration of this CS graft with the host heart without serious arrhythmia.

KW - Arrhythmia

KW - Cardiac transplantation

KW - Electrophysiology

KW - Regenerative medicine

KW - Stem cell-based therapy

UR - http://www.scopus.com/inward/record.url?scp=33645322993&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645322993&partnerID=8YFLogxK

U2 - 10.1161/01.RES.0000209515.59115.70

DO - 10.1161/01.RES.0000209515.59115.70

M3 - Article

C2 - 16469955

AN - SCOPUS:33645322993

VL - 98

SP - 705

EP - 712

JO - Circulation Research

JF - Circulation Research

SN - 0009-7330

IS - 5

ER -