PURE-LAMP procedure for the diagnosis of extrapulmonary tuberculosis: A case series

Yuji Kouzaki, Kei Mikita, Takuya Maeda, Masashi Ishihara, Shuichi Kawano, Yuji Fujikura, Kazuo Imai, Soichiro Kanoh, Kazuho Utsunomiya, Masahiro Inoue, Yasushi Miyahira, Akihiko Kawana

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Although the polymerase chain reaction is effective for the diagnosis of extrapulmonary tuberculosis (EPTB), it is typically unavailable in resource-limited situations. In contrast, the loop-mediated isothermal amplification (LAMP) assay is a relatively cost-effective and accessible method. Additionally, when combined with the procedure for ultra-rapid extraction (PURE) kit, which enables simple DNA extraction, LAMP can detect Mycobacterium tuberculosis in sputum within 1.5 hours using a simple procedure. In this study, we investigated the utility of the PURE-LAMP technique to diagnose three cases of EPTB and showed that it may potentially be a valuable tool for the diagnosis of EPTB.

Original languageEnglish
Pages (from-to)1447-1450
Number of pages4
JournalInternal Medicine
Volume54
Issue number11
DOIs
Publication statusPublished - 2015 Jun 1
Externally publishedYes

Fingerprint

Tuberculosis
Sputum
Mycobacterium tuberculosis
Costs and Cost Analysis
Polymerase Chain Reaction
DNA

Keywords

  • Diagnosis
  • Extrapulmonary tuberculosis
  • Loop-mediated isothermal amplification
  • Mycobacterium tuberculosis
  • Procedure for ultra-rapid extraction

ASJC Scopus subject areas

  • Internal Medicine
  • Medicine(all)

Cite this

Kouzaki, Y., Mikita, K., Maeda, T., Ishihara, M., Kawano, S., Fujikura, Y., ... Kawana, A. (2015). PURE-LAMP procedure for the diagnosis of extrapulmonary tuberculosis: A case series. Internal Medicine, 54(11), 1447-1450. https://doi.org/10.2169/internalmedicine.54.4615

PURE-LAMP procedure for the diagnosis of extrapulmonary tuberculosis : A case series. / Kouzaki, Yuji; Mikita, Kei; Maeda, Takuya; Ishihara, Masashi; Kawano, Shuichi; Fujikura, Yuji; Imai, Kazuo; Kanoh, Soichiro; Utsunomiya, Kazuho; Inoue, Masahiro; Miyahira, Yasushi; Kawana, Akihiko.

In: Internal Medicine, Vol. 54, No. 11, 01.06.2015, p. 1447-1450.

Research output: Contribution to journalArticle

Kouzaki, Y, Mikita, K, Maeda, T, Ishihara, M, Kawano, S, Fujikura, Y, Imai, K, Kanoh, S, Utsunomiya, K, Inoue, M, Miyahira, Y & Kawana, A 2015, 'PURE-LAMP procedure for the diagnosis of extrapulmonary tuberculosis: A case series', Internal Medicine, vol. 54, no. 11, pp. 1447-1450. https://doi.org/10.2169/internalmedicine.54.4615
Kouzaki, Yuji ; Mikita, Kei ; Maeda, Takuya ; Ishihara, Masashi ; Kawano, Shuichi ; Fujikura, Yuji ; Imai, Kazuo ; Kanoh, Soichiro ; Utsunomiya, Kazuho ; Inoue, Masahiro ; Miyahira, Yasushi ; Kawana, Akihiko. / PURE-LAMP procedure for the diagnosis of extrapulmonary tuberculosis : A case series. In: Internal Medicine. 2015 ; Vol. 54, No. 11. pp. 1447-1450.
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