Purging of acute myeloid leukemic cells by ether lipids and hyperthermia

Shinichiro Okamoto, A. C. Olson, W. E. Berdel, W. R. Vogler

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Ether lipids (EL) and hyperthermia have been shown to possess a relatively selective cytotoxicity to leukemic cells. In this study, the combined effects of EL (ET-18-OCH3, ET-16-NHCOCH3, or BM 41.440) and hyperthermia on the growth of hematopoietic progenitors, myeloid leukemic cell lines, and leukemic cells obtained from patients with acute myeloid leukemia (AML) were examined to determine if this combination resulted in a greater selective killing of leukemic cells than that achieved by either EL or heat alone. When the cells were treated simultaneously with EL (50 μg/mL) and hyperthermia (42°C) for one hour, the killing of leukemic cell line cells was enhanced considerably. Among the three EL, however, the combination of ET-18-OCH3 and heat seemed to be the most cytotoxic to leukemic cell line cells with no effect on the growth of hematopoietic progenitors. An increase in the duration of treatment with ET-18-OCH3 to four hours with heat added during the last hour resulted in a further reduction of leukemic cell line cells while sparing 50% of hematopoietic progenitors after cryopreservation. The combined treatment with ET-18-OCH3 and heat also inhibited the growth of leukemic progenitors obtained from AML patients by 97% to 100%. These data indicate that the combined treatment with EL and hyperthermia might offer an efficient means to eliminate myeloid leukemic cells in vitro.

Original languageEnglish
Pages (from-to)1777-1783
Number of pages7
JournalBlood
Volume72
Issue number5
Publication statusPublished - 1988
Externally publishedYes

Fingerprint

Purging
Myeloid Cells
Ether
Fever
Lipids
Cells
Hot Temperature
Cell Line
Acute Myeloid Leukemia
Growth
Cytotoxicity
Cryopreservation
Therapeutics
edelfosine

ASJC Scopus subject areas

  • Hematology

Cite this

Okamoto, S., Olson, A. C., Berdel, W. E., & Vogler, W. R. (1988). Purging of acute myeloid leukemic cells by ether lipids and hyperthermia. Blood, 72(5), 1777-1783.

Purging of acute myeloid leukemic cells by ether lipids and hyperthermia. / Okamoto, Shinichiro; Olson, A. C.; Berdel, W. E.; Vogler, W. R.

In: Blood, Vol. 72, No. 5, 1988, p. 1777-1783.

Research output: Contribution to journalArticle

Okamoto, S, Olson, AC, Berdel, WE & Vogler, WR 1988, 'Purging of acute myeloid leukemic cells by ether lipids and hyperthermia', Blood, vol. 72, no. 5, pp. 1777-1783.
Okamoto S, Olson AC, Berdel WE, Vogler WR. Purging of acute myeloid leukemic cells by ether lipids and hyperthermia. Blood. 1988;72(5):1777-1783.
Okamoto, Shinichiro ; Olson, A. C. ; Berdel, W. E. ; Vogler, W. R. / Purging of acute myeloid leukemic cells by ether lipids and hyperthermia. In: Blood. 1988 ; Vol. 72, No. 5. pp. 1777-1783.
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