Purification and identification of major soluble 40-kDa antigenic protein from Entamoeba histolytica: Its application for serodiagnosis of asymptomatic amebiasis

Jun Ichi Sanuki, Kyoko Nakano, Masaharu Tokoro, Tomoyoshi Nozaki, Eiichi Okuzawa, Seiki Kobayashi, Takashi Asai

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

One of the major soluble antigenic proteins of Entamoeba histolytica was purified to homogeneity and identified on a molecular basis. Its recombinant protein was expressed in Escherichia coli as a fusion protein with Shistosoma japonicum glutathione S-transferase. Apparent molecular weight of the purified antigenic protein was estimated to be 40-kDa and molecular-based analysis indicated that the purified protein was NADP+-dependent alcohol dehydrogenase (EhADH1). The application of the purified protein for the serodiagnosis of amebiasis was evaluated using an enzyme-linked immunosorbent assay applied to sera obtained from patients with amebiasis and healthy human controls. The purified protein was well recognized by the sera from asymptomatic amebiasis humans (22/22, 100%), whereas, it was less recognized by the sera from symptomatic amebiasis patients (5/16, 31%) with amebic colitis or liver abscess. To confirm the antigenicity of EhADH1, the recombinant glutathione S-transferase-EhADH1 fusion protein was also evaluated by the enzyme-linked immunosorbent assay using the same sera. The recombinant protein was also recognized by the sera from asymptomatic amebiasis humans (14/22, 64%) and less recognized by the sera from symptomatic amebiasis patients (2/16, 13%). These results suggest that the purified protein is applicable antigen for serodiagnostic screening of asymptomatic amebiasis humans.

Original languageEnglish
Pages (from-to)73-80
Number of pages8
JournalParasitology International
Volume50
Issue number2
DOIs
Publication statusPublished - 2001

Fingerprint

Amebiasis
Entamoeba histolytica
Serologic Tests
Proteins
Serum
Glutathione Transferase
Recombinant Proteins
Enzyme-Linked Immunosorbent Assay
Amoebic Dysentery
Liver Abscess
Molecular Weight
Escherichia coli
Antigens

Keywords

  • Cytosolic antigen
  • Entamoeba histolytica
  • NADP-dependent alcohol dehydrogenase
  • Serodiagnosis

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Purification and identification of major soluble 40-kDa antigenic protein from Entamoeba histolytica : Its application for serodiagnosis of asymptomatic amebiasis. / Sanuki, Jun Ichi; Nakano, Kyoko; Tokoro, Masaharu; Nozaki, Tomoyoshi; Okuzawa, Eiichi; Kobayashi, Seiki; Asai, Takashi.

In: Parasitology International, Vol. 50, No. 2, 2001, p. 73-80.

Research output: Contribution to journalArticle

Sanuki, Jun Ichi ; Nakano, Kyoko ; Tokoro, Masaharu ; Nozaki, Tomoyoshi ; Okuzawa, Eiichi ; Kobayashi, Seiki ; Asai, Takashi. / Purification and identification of major soluble 40-kDa antigenic protein from Entamoeba histolytica : Its application for serodiagnosis of asymptomatic amebiasis. In: Parasitology International. 2001 ; Vol. 50, No. 2. pp. 73-80.
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