Purification and identification of major soluble 40-kDa antigenic protein from Entamoeba histolytica: Its application for serodiagnosis of asymptomatic amebiasis

Jun Ichi Sanuki, Kyoko Nakano, Masaharu Tokoro, Tomoyoshi Nozaki, Eiichi Okuzawa, Seiki Kobayashi, Takashi Asai

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

One of the major soluble antigenic proteins of Entamoeba histolytica was purified to homogeneity and identified on a molecular basis. Its recombinant protein was expressed in Escherichia coli as a fusion protein with Shistosoma japonicum glutathione S-transferase. Apparent molecular weight of the purified antigenic protein was estimated to be 40-kDa and molecular-based analysis indicated that the purified protein was NADP+-dependent alcohol dehydrogenase (EhADH1). The application of the purified protein for the serodiagnosis of amebiasis was evaluated using an enzyme-linked immunosorbent assay applied to sera obtained from patients with amebiasis and healthy human controls. The purified protein was well recognized by the sera from asymptomatic amebiasis humans (22/22, 100%), whereas, it was less recognized by the sera from symptomatic amebiasis patients (5/16, 31%) with amebic colitis or liver abscess. To confirm the antigenicity of EhADH1, the recombinant glutathione S-transferase-EhADH1 fusion protein was also evaluated by the enzyme-linked immunosorbent assay using the same sera. The recombinant protein was also recognized by the sera from asymptomatic amebiasis humans (14/22, 64%) and less recognized by the sera from symptomatic amebiasis patients (2/16, 13%). These results suggest that the purified protein is applicable antigen for serodiagnostic screening of asymptomatic amebiasis humans.

Original languageEnglish
Pages (from-to)73-80
Number of pages8
JournalParasitology International
Volume50
Issue number2
DOIs
Publication statusPublished - 2001 Jan 1

Keywords

  • Cytosolic antigen
  • Entamoeba histolytica
  • NADP-dependent alcohol dehydrogenase
  • Serodiagnosis

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Purification and identification of major soluble 40-kDa antigenic protein from Entamoeba histolytica: Its application for serodiagnosis of asymptomatic amebiasis'. Together they form a unique fingerprint.

Cite this