Purification and tissue distribution of human thymidine phosphorylase; high expression in lymphocytes, reticulocytes and tumors

Akihiko Yoshimura, Yasuo Kuwazuru, Tatsuhiko Furukawa, Hiroki Yoshida, Kazutaka Yamada, Shin ichi Akiyama

Research output: Contribution to journalArticle

197 Citations (Scopus)

Abstract

Thymidine phosphorylase (dThdPase) is an enzyme involved in pyrimidine nucleoside metabolism, but little is known about its physiological functions. We purified dThdPase from human placenta and used it for antibody preparation. The purified material appears as a single band at 55 000 dalton on sodium dodecylsulfate-polyacrylamide gel electrophoresis. We obtained a specific antibody raised in rabbits that detected a single polypeptide with a molecular weight of 55 000 dalton in the post nuclear homogenates of several human tissues, on immunoblotting. Using the same technique, dThdPase was highly expressed in the liver, lung, spleen, lymph nodes and peripheral lymphocytes. Immunohistochemical staining revealed that macrophage-like cells contained a much higher amount of dThdPase than parenchymal cells in the liver and lung. dThdPase was found to be highly expressed in T- and B-cell-type malignant lymphoma cells, but low in lymphoblastic and myeloblastic leukemia cells. We also found that carcinomas in the stomach, colon and ovary contained higher amounts of this enzyme than non-neoplastic regions of the tissues. These data suggest that dThdPase plays a role in proliferation and/or differentiation of leukocytes and in cancer proliferation.

Original languageEnglish
Pages (from-to)107-113
Number of pages7
JournalBBA - General Subjects
Volume1034
Issue number1
DOIs
Publication statusPublished - 1990 Apr 23
Externally publishedYes

Keywords

  • (Human)
  • Immunoassay
  • Thymidine phophorylase
  • Tissue distribution

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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