Purification of a new protein stimulating RNA polymerase II from ehrlich ascites tumor cells: Comparison with proteins purified before

Yoshinobu Nakanishi, Kazuhisa Sekimizu, Hiroomi Tamura, Shunji Natori

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A protein factor named S-I(b) that stimulates the activity of RNA polymerase II was purified from Ehrlich ascites tumor cells. The final preparation gave a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This protein has a molecular weight of 24,000 and is significantly smaller than S-II and S-II', stimulatory proteins of RNA polymerase II purified previously. However, S-I(b) cross-reacted with antibody against S-II and its peptide map obtained after radio-iodination was identical with that of S-II' (a phosphorylated form of S-II), indicating that S-I(b) and S-II share a common primary structure. S-I(b) is suggested not to be a degradation product of S-II produced during the purification process, and the structural relations between S-II and S-II' are discussed.

Original languageEnglish
Pages (from-to)805-814
Number of pages10
JournalJournal of Biochemistry
Volume90
Issue number3
Publication statusPublished - 1981 Jul
Externally publishedYes

Fingerprint

Ehrlich Tumor Carcinoma
Si
RNA Polymerase II
RNA
Purification
Tumors
Tumor
Cells
Proteins
Protein
Cell
Halogenation
Sodium dodecyl sulfate
Polyacrylates
Electrophoresis
Radio
Antibodies
Sodium Dodecyl Sulfate
Peptides
Polyacrylamide Gel Electrophoresis

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Purification of a new protein stimulating RNA polymerase II from ehrlich ascites tumor cells : Comparison with proteins purified before. / Nakanishi, Yoshinobu; Sekimizu, Kazuhisa; Tamura, Hiroomi; Natori, Shunji.

In: Journal of Biochemistry, Vol. 90, No. 3, 07.1981, p. 805-814.

Research output: Contribution to journalArticle

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