Purification of Cardiac Cells

Fumiyuki Hattori, Keiichi Fukuda

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

For studies into and the clinical application of cardiomyocytes, simple methodology capable of precisely separating viable cardiomyocytes from noncardiomyocytes is required. Herein, a simple and nongenetic method is described for the separation of viable cardiomyocytes. Examples of the application of this method include (1) investigations into developmental changes in gene expression profiles in cardiomyocytes and noncardiomyocytes; (2) studies investigating drug responses of human pluripotent stem cell (PSC)-derived cardiomyocytes; (3) in vitro investigations into the interactions between cardiomyocytes and noncardiomyocytes; and (4) the use of PSC-derived cardiomyocytes in heart regenerative therapy.

Original languageEnglish
Title of host publicationMethods in Cell Biology
PublisherAcademic Press Inc.
Pages269-279
Number of pages11
DOIs
Publication statusPublished - 2012 Jan

Publication series

NameMethods in Cell Biology
Volume112
ISSN (Print)0091-679X

Keywords

  • Cardiomyocytes
  • Cell sorting
  • Differentiation
  • Embryoid bodies
  • Embryos
  • Enzymatic digestion of tissue
  • Hearts
  • Mice
  • Purification of cardiomyocytes
  • Rats
  • Ventricles

ASJC Scopus subject areas

  • Cell Biology

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