Purification of dFMR1-containing complexes using tandem affinity purification

Keita Miyoshi, Akiyo Ogino, Mikiko C. Siomi, Haruhiko Siomi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Fragile X syndrome results from the lack of FMR1 expression. To understand how the lack of FMR1 function leads to the syndrome, we are studying the Drosophila FMR1 related protein (dFMR1). We performed affinity purification of dFMR1-associated complexes from cultured Drosophila S2 cells and found that dFMR1 associates with a key component of RNA interference, AGO2. Our finding suggests cross talk between the fragile X syndrome protein and RNA interference. In this chapter, we describe a tandem affinity purification method to isolate the protein components and small RNAs in the dFMR1-associated complexes.

Original languageEnglish
Title of host publicationTrinucleotide Repeat Protocols
Pages111-121
Number of pages11
DOIs
Publication statusPublished - 2013 Jul 24

Publication series

NameMethods in Molecular Biology
Volume1010
ISSN (Print)1064-3745

Keywords

  • Argonaute
  • Drosophila
  • Fragile X mental retardation
  • RNA silencing
  • Small RNA
  • Tandem affinity purification
  • dFMR1

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Miyoshi, K., Ogino, A., Siomi, M. C., & Siomi, H. (2013). Purification of dFMR1-containing complexes using tandem affinity purification. In Trinucleotide Repeat Protocols (pp. 111-121). (Methods in Molecular Biology; Vol. 1010). https://doi.org/10.1007/978-1-62703-411-1-8