Purification of two transcription factors required for initiation by mammalian RNA polymerase II

Tatsuo Kawaguchi, Shigetaka Kitajima, Yoshiyuki Niho, Taiko Oda, Joseph Germino, Sherman M. Weissman, Yukio Yasukochi

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Abstract

We have purified two general transcription factors (FA and FE) necessary for specific transcription by mammalian RNA polymerase II to near homogeneity. Both activities are associated with peptides of ≈33 kDa. FA and FE do not replace one another and show different kinetics of action in a sarkosyl block assay. In particular, FE participated in a rapid reaction after the formation of an initial complex with the other transcription factors. Furthermore, FE can associate with purified calf thymus RNA polymerase II.

Original languageEnglish
Pages (from-to)6619-6623
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number17
Publication statusPublished - 1990 Sep

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ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Kawaguchi, T., Kitajima, S., Niho, Y., Oda, T., Germino, J., Weissman, S. M., & Yasukochi, Y. (1990). Purification of two transcription factors required for initiation by mammalian RNA polymerase II. Proceedings of the National Academy of Sciences of the United States of America, 87(17), 6619-6623.