Purification of two transcription factors required for initiation by mammalian RNA polymerase II

Tatsuo Kawaguchi, Shigetaka Kitajima, Yoshiyuki Niho, Taiko Oda, Joseph Germino, Sherman M. Weissman, Yukio Yasukochi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We have purified two general transcription factors (FA and FE) necessary for specific transcription by mammalian RNA polymerase II to near homogeneity. Both activities are associated with peptides of ≈33 kDa. FA and FE do not replace one another and show different kinetics of action in a sarkosyl block assay. In particular, FE participated in a rapid reaction after the formation of an initial complex with the other transcription factors. Furthermore, FE can associate with purified calf thymus RNA polymerase II.

Original languageEnglish
Pages (from-to)6619-6623
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number17
Publication statusPublished - 1990 Sep

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RNA Polymerase II
Transcription Factors
General Transcription Factors
Thymus Gland
Peptides
sarkosyl

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Kawaguchi, T., Kitajima, S., Niho, Y., Oda, T., Germino, J., Weissman, S. M., & Yasukochi, Y. (1990). Purification of two transcription factors required for initiation by mammalian RNA polymerase II. Proceedings of the National Academy of Sciences of the United States of America, 87(17), 6619-6623.

Purification of two transcription factors required for initiation by mammalian RNA polymerase II. / Kawaguchi, Tatsuo; Kitajima, Shigetaka; Niho, Yoshiyuki; Oda, Taiko; Germino, Joseph; Weissman, Sherman M.; Yasukochi, Yukio.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 17, 09.1990, p. 6619-6623.

Research output: Contribution to journalArticle

Kawaguchi, T, Kitajima, S, Niho, Y, Oda, T, Germino, J, Weissman, SM & Yasukochi, Y 1990, 'Purification of two transcription factors required for initiation by mammalian RNA polymerase II', Proceedings of the National Academy of Sciences of the United States of America, vol. 87, no. 17, pp. 6619-6623.
Kawaguchi, Tatsuo ; Kitajima, Shigetaka ; Niho, Yoshiyuki ; Oda, Taiko ; Germino, Joseph ; Weissman, Sherman M. ; Yasukochi, Yukio. / Purification of two transcription factors required for initiation by mammalian RNA polymerase II. In: Proceedings of the National Academy of Sciences of the United States of America. 1990 ; Vol. 87, No. 17. pp. 6619-6623.
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