Purified Human Dental Pulp Stem Cells Promote Osteogenic Regeneration

T. Yasui, Y. Mabuchi, H. Toriumi, T. Ebine, K. Niibe, D. D. Houlihan, Satoru Morikawa, K. Onizawa, Hiromasa Kawana, C. Akazawa, N. Suzuki, Taneaki Nakagawa, Hideyuki Okano, Y. Matsuzaki

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Human dental pulp stem/progenitor cells (hDPSCs) are attractive candidates for regenerative therapy because they can be easily expanded to generate colony-forming unit-fibroblasts (CFU-Fs) on plastic and the large cell numbers required for transplantation. However, isolation based on adherence to plastic inevitably changes the surface marker expression and biological properties of the cells. Consequently, little is currently known about the original phenotypes of tissue precursor cells that give rise to plastic-adherent CFU-Fs. To better understand the in vivo functions and translational therapeutic potential of hDPSCs and other stem cells, selective cell markers must be identified in the progenitor cells. Here, we identified a dental pulp tissue-specific cell population based on the expression profiles of 2 cell-surface markers LNGFR (CD271) and THY-1 (CD90). Prospectively isolated, dental pulp-derived LNGFRLow+THY-1High+ cells represent a highly enriched population of clonogenic cells - notably, the isolated cells exhibited long-term proliferation and multilineage differentiation potential in vitro. The cells also expressed known mesenchymal cell markers and promoted new bone formation to heal critical-size calvarial defects in vivo. These findings suggest that LNGFRLow+THY-1High+ dental pulp-derived cells provide an excellent source of material for bone regenerative strategies.

Original languageEnglish
Pages (from-to)206-214
Number of pages9
JournalJournal of Dental Research
Volume95
Issue number2
DOIs
Publication statusPublished - 2016 Feb 1

Fingerprint

Dental Pulp
Regeneration
Stem Cells
Plastics
Osteogenesis
Population
Fibroblasts
Cell Count
Transplantation
Biomarkers

Keywords

  • bone regeneration
  • flow cytometry
  • isolation
  • low-affinity nerve growth factor receptor
  • THY-1
  • transplantation

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Yasui, T., Mabuchi, Y., Toriumi, H., Ebine, T., Niibe, K., Houlihan, D. D., ... Matsuzaki, Y. (2016). Purified Human Dental Pulp Stem Cells Promote Osteogenic Regeneration. Journal of Dental Research, 95(2), 206-214. https://doi.org/10.1177/0022034515610748

Purified Human Dental Pulp Stem Cells Promote Osteogenic Regeneration. / Yasui, T.; Mabuchi, Y.; Toriumi, H.; Ebine, T.; Niibe, K.; Houlihan, D. D.; Morikawa, Satoru; Onizawa, K.; Kawana, Hiromasa; Akazawa, C.; Suzuki, N.; Nakagawa, Taneaki; Okano, Hideyuki; Matsuzaki, Y.

In: Journal of Dental Research, Vol. 95, No. 2, 01.02.2016, p. 206-214.

Research output: Contribution to journalArticle

Yasui, T, Mabuchi, Y, Toriumi, H, Ebine, T, Niibe, K, Houlihan, DD, Morikawa, S, Onizawa, K, Kawana, H, Akazawa, C, Suzuki, N, Nakagawa, T, Okano, H & Matsuzaki, Y 2016, 'Purified Human Dental Pulp Stem Cells Promote Osteogenic Regeneration', Journal of Dental Research, vol. 95, no. 2, pp. 206-214. https://doi.org/10.1177/0022034515610748
Yasui T, Mabuchi Y, Toriumi H, Ebine T, Niibe K, Houlihan DD et al. Purified Human Dental Pulp Stem Cells Promote Osteogenic Regeneration. Journal of Dental Research. 2016 Feb 1;95(2):206-214. https://doi.org/10.1177/0022034515610748
Yasui, T. ; Mabuchi, Y. ; Toriumi, H. ; Ebine, T. ; Niibe, K. ; Houlihan, D. D. ; Morikawa, Satoru ; Onizawa, K. ; Kawana, Hiromasa ; Akazawa, C. ; Suzuki, N. ; Nakagawa, Taneaki ; Okano, Hideyuki ; Matsuzaki, Y. / Purified Human Dental Pulp Stem Cells Promote Osteogenic Regeneration. In: Journal of Dental Research. 2016 ; Vol. 95, No. 2. pp. 206-214.
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