Purified interleukin 5 supports the terminal differentiation and proliferation of murine eosinophilic precursors

Y. Yamaguchi, T. Suda, J. Suda, M. Eguchi, Y. Miura, N. Harada, A. Tominaga, K. Takatsu

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Abstract

Using a clonal culture system, we investigated the hemopoietic effects of purified recombinant IL-5 obtained from conditioned media of transfected Xenopus oocytes. IL-5 alone acted on untreated bone marrow cells and supported the formation of a small number of colonies, all of which were predominantly eosinophilic. However, it did not support colony formation by spleen cells from 5-FU-treated mice, in which only primitive stem cells had survived, while Il-3 and G-CSF did. Eosinophil-containing colonies were formed from these cells in the presence of IL-5 and G-CSF together. In contrast, G-CSF alone did not support any eosinophil colonies. The eosinophilopoietic effect of IL-5 was dose-dependent, and was neutralized specifically by anti-IL-5 antibody. To exclude the possibility of interactions with accessory cells in the same culture dish, we replated a small number (200 cells/dish) of enriched hemopoietic progenitors, obtained from blast cell colonies, which were formed by cultivation of spleen cells from 5-FU-treated mice in the presence of IL-3 or G-CSF. From these replated blast cells, eosinophil colonies were induced in dishes containing IL-5 but not in those containing G-CSF alone. From these findings, it was concluded that IL-5 did not act on primitive hemopoietic cells, but on blast cells induced by IL-3 orG-CSF. IL-5 specifically facilitated the terminal differentiation and proliferation of eosinophils. In this respect, the role of IL-5 in eosinophilopoiesis seems to be analogous to erythropoietin, which promotes the terminal differentiation and amplification of erythroid cells. Moreover, IL-5 maintained the viability of mature eosinophils obtained from peritoneal exudate cells of the mice infected with parasites, indicating mature functional eosinophils carried IL-5 receptors. The synergistic effects of Il-5 and colony-stimulting factors on the expansion of eosinophils is supposed to contribute to the urgent mobilization of eosinophils at the time of helminthic infections and allergic responses.

Original languageEnglish
Pages (from-to)43-56
Number of pages14
JournalJournal of Experimental Medicine
Volume167
Issue number1
Publication statusPublished - 1988

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Interleukin-5
Eosinophils
Granulocyte Colony-Stimulating Factor
Interleukin-3
Fluorouracil
Interleukin-5 Receptors
Spleen
Erythroid Cells
Exudates and Transudates
Conditioned Culture Medium
Erythropoietin
Xenopus
Bone Marrow Cells
Oocytes
Parasites
Stem Cells
Cell Count
Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Yamaguchi, Y., Suda, T., Suda, J., Eguchi, M., Miura, Y., Harada, N., ... Takatsu, K. (1988). Purified interleukin 5 supports the terminal differentiation and proliferation of murine eosinophilic precursors. Journal of Experimental Medicine, 167(1), 43-56.

Purified interleukin 5 supports the terminal differentiation and proliferation of murine eosinophilic precursors. / Yamaguchi, Y.; Suda, T.; Suda, J.; Eguchi, M.; Miura, Y.; Harada, N.; Tominaga, A.; Takatsu, K.

In: Journal of Experimental Medicine, Vol. 167, No. 1, 1988, p. 43-56.

Research output: Contribution to journalArticle

Yamaguchi, Y, Suda, T, Suda, J, Eguchi, M, Miura, Y, Harada, N, Tominaga, A & Takatsu, K 1988, 'Purified interleukin 5 supports the terminal differentiation and proliferation of murine eosinophilic precursors', Journal of Experimental Medicine, vol. 167, no. 1, pp. 43-56.
Yamaguchi, Y. ; Suda, T. ; Suda, J. ; Eguchi, M. ; Miura, Y. ; Harada, N. ; Tominaga, A. ; Takatsu, K. / Purified interleukin 5 supports the terminal differentiation and proliferation of murine eosinophilic precursors. In: Journal of Experimental Medicine. 1988 ; Vol. 167, No. 1. pp. 43-56.
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