Purified mesenchymal stem cells are an efficient source for iPS cell induction

Kunimichi Niibe, Yoshimi Kawamura, Daisuke Araki, Satoru Morikawa, Kyoko Miura, Sadafumi Suzuki, Shigeto Shimmura, Takehiko Sunabori, Yo Mabuchi, Yasuo Nagai, Taneaki Nakagawa, Hideyuki Okano, Yumi Matsuzaki

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Background: Induced pluripotent stem (iPS) cells are generated from mouse and human somatic cells by the forced expression of defined transcription factors. Although most somatic cells are capable of acquiring pluripotency with minimal gene transduction, the poor efficiency of cell reprogramming and the uneven quality of iPS cells are still important problems. In particular, the choice of cell type most suitable for inducing high-quality iPS cells remains unclear. Methodology/Principal Findings: Here, we generated iPS cells from PDGFRα+ Sca-1+ (PαS) adult mouse mesenchymal stem cells (MSCs) and PDGFRα- Sca-1- osteo-progenitors (OP cells), and compared the induction efficiency and quality of individual iPS clones. MSCs had a higher reprogramming efficiency compared with OP cells and Tail Tip Fibroblasts (TTFs). The iPS cells induced from MSCs by Oct3/4, Sox2, and Klf4 appeared to be the closest equivalent to ES cells by DNA microarray gene profile and germline-transmission efficiency. Conclusions/Significance: Our findings suggest that a purified source of undifferentiated cells from adult tissue can produce high-quality iPS cells. In this context, prospectively enriched MSCs are a promising candidate for the efficient generation of high-quality iPS cells.

Original languageEnglish
Article numbere17610
JournalPLoS One
Volume6
Issue number3
DOIs
Publication statusPublished - 2011

Fingerprint

Induced Pluripotent Stem Cells
Stem cells
Mesenchymal Stromal Cells
stem cells
somatic cells
cells
mice
induced pluripotent stem cells
Oligonucleotide Array Sequence Analysis
Genes
fibroblasts
Tail
germ cells
Transcription Factors
tail
Stem Cells
genes
transcription factors
Clone Cells
Fibroblasts

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Niibe, K., Kawamura, Y., Araki, D., Morikawa, S., Miura, K., Suzuki, S., ... Matsuzaki, Y. (2011). Purified mesenchymal stem cells are an efficient source for iPS cell induction. PLoS One, 6(3), [e17610]. https://doi.org/10.1371/journal.pone.0017610

Purified mesenchymal stem cells are an efficient source for iPS cell induction. / Niibe, Kunimichi; Kawamura, Yoshimi; Araki, Daisuke; Morikawa, Satoru; Miura, Kyoko; Suzuki, Sadafumi; Shimmura, Shigeto; Sunabori, Takehiko; Mabuchi, Yo; Nagai, Yasuo; Nakagawa, Taneaki; Okano, Hideyuki; Matsuzaki, Yumi.

In: PLoS One, Vol. 6, No. 3, e17610, 2011.

Research output: Contribution to journalArticle

Niibe, K, Kawamura, Y, Araki, D, Morikawa, S, Miura, K, Suzuki, S, Shimmura, S, Sunabori, T, Mabuchi, Y, Nagai, Y, Nakagawa, T, Okano, H & Matsuzaki, Y 2011, 'Purified mesenchymal stem cells are an efficient source for iPS cell induction', PLoS One, vol. 6, no. 3, e17610. https://doi.org/10.1371/journal.pone.0017610
Niibe, Kunimichi ; Kawamura, Yoshimi ; Araki, Daisuke ; Morikawa, Satoru ; Miura, Kyoko ; Suzuki, Sadafumi ; Shimmura, Shigeto ; Sunabori, Takehiko ; Mabuchi, Yo ; Nagai, Yasuo ; Nakagawa, Taneaki ; Okano, Hideyuki ; Matsuzaki, Yumi. / Purified mesenchymal stem cells are an efficient source for iPS cell induction. In: PLoS One. 2011 ; Vol. 6, No. 3.
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