Purified opioid μ-receptor is of a different molecular size than δ- and κ-receptors

Hiroshi Ueda, Hitoshi Harada, Hidemi Misawa, Masakatsu Nozaki, Hiroshi Takagi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Opioid μ-receptors were solubilized from rat brains with 0.5% Triton X-100 in the presence of 0.32 M sucrose and then purified with 6-succinyl morphine-Affi-Gel 102 column. Purified materials showed a major band with mol.wt. 58,000 Da and a minor band with mol.wt. 41,000 Da. Affinity cross linking with [3H]DAGO, a selective μ-opioid agonist, to the neural membranes and purified materials revealed that opioid μ-binding protein has a mol.wt. of 58,000 Da, a value totally different from that of δ- (mol.wt. 18,500 Da) and κ- (mol.wt. 36,600 Da) binding protein.

Original languageEnglish
Pages (from-to)339-344
Number of pages6
JournalNeuroscience Letters
Volume75
Issue number3
DOIs
Publication statusPublished - 1987 Apr 10
Externally publishedYes

Fingerprint

Opioid Receptors
Opioid Analgesics
Carrier Proteins
Ala(2)-MePhe(4)-Gly(5)-enkephalin
Octoxynol
Morphine
Sucrose
Gels
Membranes
Brain

Keywords

  • 6-Succinyl morphine-Affi-Gel 102
  • Cross linking
  • Opioid μ-receptor
  • Rat brain

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Purified opioid μ-receptor is of a different molecular size than δ- and κ-receptors. / Ueda, Hiroshi; Harada, Hitoshi; Misawa, Hidemi; Nozaki, Masakatsu; Takagi, Hiroshi.

In: Neuroscience Letters, Vol. 75, No. 3, 10.04.1987, p. 339-344.

Research output: Contribution to journalArticle

Ueda, Hiroshi ; Harada, Hitoshi ; Misawa, Hidemi ; Nozaki, Masakatsu ; Takagi, Hiroshi. / Purified opioid μ-receptor is of a different molecular size than δ- and κ-receptors. In: Neuroscience Letters. 1987 ; Vol. 75, No. 3. pp. 339-344.
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