Purinergic signaling promotes proliferation of adult mouse subventricular zone cells

Satoshi Suyama, Takehiko Sunabori, Hiroaki Kanki, Kazunobu Sawamoto, Christian Gachet, Schuichi Koizumi, Hideyuki Okano

Research output: Contribution to journalArticle

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Abstract

In adult mammalian brains, neural stem cells (NSCs) exist in the subventricular zone (SVZ), where persistent neurogenesis continues throughout life. Those NSCs produce neuroblasts that migrate into the olfactory bulb via formation of transit-amplifying cells, which are committed precursor cells of the neuronal lineage. In this SVZ niche, cell-cell communications conducted by diffusible factors as well as physical cell-cell contacts are important for the regulation of the proliferation and fate determination of NSCs. Previous studies have suggested that extracellular purinergic signaling, which is mediated by purine compounds such as ATP, plays important roles in cell-cell communication in the CNS. Purinergic signaling also promotes the proliferation of adult NSCs in vitro. However, the in vivo roles of purinergic signaling in the neurogenic niche still remain unknown. In this study, ATP infusion into the lateral ventricle of the mouse brain resulted in an increase in the numbers of rapidly dividing cells and Mash1-positive transit-amplifying cells (Type C cells) in the SVZ. Mash1-positive cells express the P2Y1 purinergic signaling receptor and infusion of the P2Y1 receptor-specific antagonist MRS2179 decreased the number of rapidly dividing bromodeoxyuridine (BrdU)-positive cells and Type C cells. Moreover, a 17% reduction of rapidly dividing BrdU-positive cells and a 19% reduction of Mash1-positive cells were observed in P2Y1 knock-out mice. Together, these results suggest that purinergic signaling promotes the proliferation of rapidly dividing cells and transit-amplifying cells, in the SVZ niche through the P2Y1 receptor.

Original languageEnglish
Pages (from-to)9238-9247
Number of pages10
JournalJournal of Neuroscience
Volume32
Issue number27
DOIs
Publication statusPublished - 2012 Jul 4

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Lateral Ventricles
Neural Stem Cells
Purinergic P2Y1 Receptors
Bromodeoxyuridine
Cell Communication
Adenosine Triphosphate
Adult Stem Cells
Olfactory Bulb
Neurogenesis
Cell Lineage
Knockout Mice
Brain Stem

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Purinergic signaling promotes proliferation of adult mouse subventricular zone cells. / Suyama, Satoshi; Sunabori, Takehiko; Kanki, Hiroaki; Sawamoto, Kazunobu; Gachet, Christian; Koizumi, Schuichi; Okano, Hideyuki.

In: Journal of Neuroscience, Vol. 32, No. 27, 04.07.2012, p. 9238-9247.

Research output: Contribution to journalArticle

Suyama, S, Sunabori, T, Kanki, H, Sawamoto, K, Gachet, C, Koizumi, S & Okano, H 2012, 'Purinergic signaling promotes proliferation of adult mouse subventricular zone cells', Journal of Neuroscience, vol. 32, no. 27, pp. 9238-9247. https://doi.org/10.1523/JNEUROSCI.4001-11.2012
Suyama S, Sunabori T, Kanki H, Sawamoto K, Gachet C, Koizumi S et al. Purinergic signaling promotes proliferation of adult mouse subventricular zone cells. Journal of Neuroscience. 2012 Jul 4;32(27):9238-9247. https://doi.org/10.1523/JNEUROSCI.4001-11.2012
Suyama, Satoshi ; Sunabori, Takehiko ; Kanki, Hiroaki ; Sawamoto, Kazunobu ; Gachet, Christian ; Koizumi, Schuichi ; Okano, Hideyuki. / Purinergic signaling promotes proliferation of adult mouse subventricular zone cells. In: Journal of Neuroscience. 2012 ; Vol. 32, No. 27. pp. 9238-9247.
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