Putidaredoxin-cytochrome P450(cam) interaction: Spin state of the heme iron modulates putidaredoxin structure

Hideo Shimada, Shingo Nagano, Yoko Ariga, Masashi Unno, Tsuyoshi Egawa, Takako Hishiki, Yuzuru Ishimura, Futoshi Masuya, Takashi Obata, Hiroshi Hori

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

During the monooxygenase reaction catalyzed by cytochrome P450(cam) (P450(cam)), a ternary complex of P450(cam), reduced putidaredoxin, and d- camphor is formed as an obligatory reaction intermediate. When ligands such as CO, NO, and O2 bind to the heme iron of P450(cam) in the intermediate complex, the EPR spectrum of reduced putidaredoxin with a characteristic signal at 346 millitesla at 77 K changed into a spectrum having a new signal at 348 millitesla. The experiment with O2 was carried out by employing a mutant P450(cam) with Asp251 → Asn or Gly where the rate of electron transfer from putidaredoxin to oxyferrous P450(cam) is considerably reduced. Such a ligand-induced EPR spectral change of putidaredoxin was also shown in situ in Pseudomonas putida. Mutations introduced into the neighborhood of the iron-sulfur cluster of putidaredoxin revealed that a Ser44 → Gly mutation mimicked the ligand-induced spectral change of putidaredoxin. Arg109 and Arg112, which are in the putative putidaredoxin binding site of P450(cam), were essential for the spectral changes of putidaredoxin in the complex. These results indicate that a change in the P450(cam) active site that is the consequence of an altered spin state is transmitted to putidaredoxin within the ternary complex and produces a conformational change of the 2Fe-2S active center.

Original languageEnglish
Pages (from-to)9363-9369
Number of pages7
JournalJournal of Biological Chemistry
Volume274
Issue number14
DOIs
Publication statusPublished - 1999 Apr 2

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Camphor 5-Monooxygenase
Heme
Cams
Iron
Ligands
Paramagnetic resonance
Camphor
putidaredoxin
Reaction intermediates
Pseudomonas putida
Mutation
Carbon Monoxide
Mixed Function Oxygenases
Sulfur
Catalytic Domain
Binding Sites

ASJC Scopus subject areas

  • Biochemistry

Cite this

Putidaredoxin-cytochrome P450(cam) interaction : Spin state of the heme iron modulates putidaredoxin structure. / Shimada, Hideo; Nagano, Shingo; Ariga, Yoko; Unno, Masashi; Egawa, Tsuyoshi; Hishiki, Takako; Ishimura, Yuzuru; Masuya, Futoshi; Obata, Takashi; Hori, Hiroshi.

In: Journal of Biological Chemistry, Vol. 274, No. 14, 02.04.1999, p. 9363-9369.

Research output: Contribution to journalArticle

Shimada, H, Nagano, S, Ariga, Y, Unno, M, Egawa, T, Hishiki, T, Ishimura, Y, Masuya, F, Obata, T & Hori, H 1999, 'Putidaredoxin-cytochrome P450(cam) interaction: Spin state of the heme iron modulates putidaredoxin structure', Journal of Biological Chemistry, vol. 274, no. 14, pp. 9363-9369. https://doi.org/10.1074/jbc.274.14.9363
Shimada, Hideo ; Nagano, Shingo ; Ariga, Yoko ; Unno, Masashi ; Egawa, Tsuyoshi ; Hishiki, Takako ; Ishimura, Yuzuru ; Masuya, Futoshi ; Obata, Takashi ; Hori, Hiroshi. / Putidaredoxin-cytochrome P450(cam) interaction : Spin state of the heme iron modulates putidaredoxin structure. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 14. pp. 9363-9369.
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