Pyrrolidinium-type fullerene derivative-induced apoptosis by the generation of reactive oxygen species in HL-60 cells

Chiho Nishizawa, Noriyuki Hashimoto, Sachiko Yokoo, Megumi Funakoshi-Tago, Tadashi Kasahara, Kyoko Takahashi, Shigeo Nakamura, Tadahiko Mashino

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The biological activities of C60-bis(N,N-dimethylpyrrolidinium iodide), a water-soluble cationic fullerene derivative, on human promyeloleukaemia (HL-60) cells were investigated. The pyrrolidinium fullerene derivative showed cytotoxicity in HL-60 cells. The characteristics of apoptosis, such as DNA fragmentation and condensation of chromatin in HL-60 cells, were observed by exposure to the pyrrolidinium fullerene derivative. Caspase-3 and-8 were activated and cytochrome c was also released from mitochondria. The generation of reactive oxygen species (ROS) by the pyrrolidinium fullerene derivative was observed by DCFH-DA, a fluorescence probe for the detection of ROS. Pre-treatment with α-tocopherol suppressed cell death and intracellular oxidative stress caused by the pyrrolidinium fullerene derivative. The apoptotic cell death induced by the pyrrolidinium fullerene derivative was suggested to be mediated by ROS generated by the pyrrolidinium fullerene derivative.

Original languageEnglish
Pages (from-to)1240-1247
Number of pages8
JournalFree Radical Research
Volume43
Issue number12
DOIs
Publication statusPublished - 2009

Keywords

  • Apoptosis
  • Caspase
  • Fullerene derivative
  • HL-60
  • Reactive oxygen species

ASJC Scopus subject areas

  • Biochemistry

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