Pyruvate kinase type-II isozyme in Plasmodium falciparum localizes to the apicoplast

Takuya Maeda, Tomoya Saito, Omar S. Harb, David S. Roos, Satoru Takeo, Hiroko Suzuki, Takafumi Tsuboi, Tsutomu Takeuchi, Takashi Asai

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Bioinformatics research on Plasmodium falciparum revealed two isoforms of pyruvate kinase: type-I and type-II enzymes. The type-I enzyme shows typical glycolytic properties, while type-II enzyme is involved in fatty acid type-II biosynthesis and has been predicted to localize to the apicoplast with the targeting signal in its N-terminus. The type-I and type-II isoforms have the same evolutionary origin as Toxoplasma gondii isozymes, TgPyKI and TgPyKII, respectively; however, TgPyKII localizes to both the mitochondrion and the apicoplast. Accordingly, we made a recombinant full length of P. falciparum pyruvate kinase type-II protein using a wheat germ cell-free expression system and obtained a specific antibody against the type-II protein. Fluorescent microscopic analysis revealed that P. falciparum type-II enzyme was localized only to the apicoplast, not to the mitochondrion. The data suggest differences in localization and metabolic pathways between P. falciparum and T. gondii pyruvate kinase isoforms.

Original languageEnglish
Pages (from-to)101-105
Number of pages5
JournalParasitology International
Volume58
Issue number1
DOIs
Publication statusPublished - 2009 Mar

Fingerprint

Apicoplasts
Pyruvate Kinase
Plasmodium falciparum
Isoenzymes
Protein Isoforms
Toxoplasma
Enzymes
Mitochondria
Cell-Free System
Metabolic Networks and Pathways
Computational Biology
Germ Cells
Triticum
Proteins
Fatty Acids
Antibodies
Research

Keywords

  • Apicoplast
  • Cell-free expression
  • Mitochondria
  • Plasmodium falciparum
  • Pyruvate kinase II

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Maeda, T., Saito, T., Harb, O. S., Roos, D. S., Takeo, S., Suzuki, H., ... Asai, T. (2009). Pyruvate kinase type-II isozyme in Plasmodium falciparum localizes to the apicoplast. Parasitology International, 58(1), 101-105. https://doi.org/10.1016/j.parint.2008.10.005

Pyruvate kinase type-II isozyme in Plasmodium falciparum localizes to the apicoplast. / Maeda, Takuya; Saito, Tomoya; Harb, Omar S.; Roos, David S.; Takeo, Satoru; Suzuki, Hiroko; Tsuboi, Takafumi; Takeuchi, Tsutomu; Asai, Takashi.

In: Parasitology International, Vol. 58, No. 1, 03.2009, p. 101-105.

Research output: Contribution to journalArticle

Maeda, T, Saito, T, Harb, OS, Roos, DS, Takeo, S, Suzuki, H, Tsuboi, T, Takeuchi, T & Asai, T 2009, 'Pyruvate kinase type-II isozyme in Plasmodium falciparum localizes to the apicoplast', Parasitology International, vol. 58, no. 1, pp. 101-105. https://doi.org/10.1016/j.parint.2008.10.005
Maeda, Takuya ; Saito, Tomoya ; Harb, Omar S. ; Roos, David S. ; Takeo, Satoru ; Suzuki, Hiroko ; Tsuboi, Takafumi ; Takeuchi, Tsutomu ; Asai, Takashi. / Pyruvate kinase type-II isozyme in Plasmodium falciparum localizes to the apicoplast. In: Parasitology International. 2009 ; Vol. 58, No. 1. pp. 101-105.
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