Quad Lipid Bilayer Module with 1-Gω Series Resistors Toward Quantitative Stochastic-Biosensors

Yoshihisa Ito, Toshihisa Osaki, Koki Kamiya, Tetsuya Yamada, Norihisa Miki, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This work designs and demonstrates the lipid bilayer-based ultra-sensitive biosensor capable of short-time detection in a quantitative manner, which is achieved by connecting multiple sensor elements in parallel.Artificial cell membranes with functional membrane proteins can form sensitive/selective biosensors. But the sensors suffer lengthy detection time at low concentration of analytes because the sensing mechanism relies on stochastic phenomena.In this work, we connected independent membrane sensors in parallel, where the ionic current through the multiple membranes was monitored by a single detector. With this format, the detection time is shortened based on the number of the array and the sensing can be quantitative. We developed a quad sensor assembling four membranes in parallel, and examined detection time of a single membrane sensor, the quad sensor, and four parallel quad-sensors. 1-Gω resistor was installed in series with each membrane to avoid overload current caused by membrane rupture. The results showed significant improvement in the detection time and deviation by the parallelization, which promises quantitative monitoring with the stochastic sensor.

Original languageEnglish
Title of host publication2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages6-8
Number of pages3
ISBN (Electronic)9781728120072
DOIs
Publication statusPublished - 2019 Jun 1
Event20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII - Berlin, Germany
Duration: 2019 Jun 232019 Jun 27

Publication series

Name2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII

Conference

Conference20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
CountryGermany
CityBerlin
Period19/6/2319/6/27

Fingerprint

Lipid Bilayer
Lipid bilayers
Biosensor
bioinstrumentation
Biosensors
resistors
Resistors
lipids
modules
Membrane
Module
Sensor
membranes
Membranes
Series
sensors
Sensors
Sensing
Membrane Protein
Elvitegravir, Cobicistat, Emtricitabine, Tenofovir Disoproxil Fumarate Drug Combination

Keywords

  • Artificial cell membranes
  • Nanopore
  • Parallelization
  • Rapid detection
  • Stochastic biosensors

ASJC Scopus subject areas

  • Process Chemistry and Technology
  • Spectroscopy
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Electronic, Optical and Magnetic Materials
  • Control and Optimization
  • Instrumentation

Cite this

Ito, Y., Osaki, T., Kamiya, K., Yamada, T., Miki, N., & Takeuchi, S. (2019). Quad Lipid Bilayer Module with 1-Gω Series Resistors Toward Quantitative Stochastic-Biosensors. In 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII (pp. 6-8). [8808191] (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2019.8808191

Quad Lipid Bilayer Module with 1-Gω Series Resistors Toward Quantitative Stochastic-Biosensors. / Ito, Yoshihisa; Osaki, Toshihisa; Kamiya, Koki; Yamada, Tetsuya; Miki, Norihisa; Takeuchi, Shoji.

2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc., 2019. p. 6-8 8808191 (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ito, Y, Osaki, T, Kamiya, K, Yamada, T, Miki, N & Takeuchi, S 2019, Quad Lipid Bilayer Module with 1-Gω Series Resistors Toward Quantitative Stochastic-Biosensors. in 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII., 8808191, 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII, Institute of Electrical and Electronics Engineers Inc., pp. 6-8, 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII, Berlin, Germany, 19/6/23. https://doi.org/10.1109/TRANSDUCERS.2019.8808191
Ito Y, Osaki T, Kamiya K, Yamada T, Miki N, Takeuchi S. Quad Lipid Bilayer Module with 1-Gω Series Resistors Toward Quantitative Stochastic-Biosensors. In 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc. 2019. p. 6-8. 8808191. (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII). https://doi.org/10.1109/TRANSDUCERS.2019.8808191
Ito, Yoshihisa ; Osaki, Toshihisa ; Kamiya, Koki ; Yamada, Tetsuya ; Miki, Norihisa ; Takeuchi, Shoji. / Quad Lipid Bilayer Module with 1-Gω Series Resistors Toward Quantitative Stochastic-Biosensors. 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc., 2019. pp. 6-8 (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII).
@inproceedings{1de6e8dd1bdc4e779104b3d48d533d1d,
title = "Quad Lipid Bilayer Module with 1-Gω Series Resistors Toward Quantitative Stochastic-Biosensors",
abstract = "This work designs and demonstrates the lipid bilayer-based ultra-sensitive biosensor capable of short-time detection in a quantitative manner, which is achieved by connecting multiple sensor elements in parallel.Artificial cell membranes with functional membrane proteins can form sensitive/selective biosensors. But the sensors suffer lengthy detection time at low concentration of analytes because the sensing mechanism relies on stochastic phenomena.In this work, we connected independent membrane sensors in parallel, where the ionic current through the multiple membranes was monitored by a single detector. With this format, the detection time is shortened based on the number of the array and the sensing can be quantitative. We developed a quad sensor assembling four membranes in parallel, and examined detection time of a single membrane sensor, the quad sensor, and four parallel quad-sensors. 1-Gω resistor was installed in series with each membrane to avoid overload current caused by membrane rupture. The results showed significant improvement in the detection time and deviation by the parallelization, which promises quantitative monitoring with the stochastic sensor.",
keywords = "Artificial cell membranes, Nanopore, Parallelization, Rapid detection, Stochastic biosensors",
author = "Yoshihisa Ito and Toshihisa Osaki and Koki Kamiya and Tetsuya Yamada and Norihisa Miki and Shoji Takeuchi",
year = "2019",
month = "6",
day = "1",
doi = "10.1109/TRANSDUCERS.2019.8808191",
language = "English",
series = "2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
pages = "6--8",
booktitle = "2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII",

}

TY - GEN

T1 - Quad Lipid Bilayer Module with 1-Gω Series Resistors Toward Quantitative Stochastic-Biosensors

AU - Ito, Yoshihisa

AU - Osaki, Toshihisa

AU - Kamiya, Koki

AU - Yamada, Tetsuya

AU - Miki, Norihisa

AU - Takeuchi, Shoji

PY - 2019/6/1

Y1 - 2019/6/1

N2 - This work designs and demonstrates the lipid bilayer-based ultra-sensitive biosensor capable of short-time detection in a quantitative manner, which is achieved by connecting multiple sensor elements in parallel.Artificial cell membranes with functional membrane proteins can form sensitive/selective biosensors. But the sensors suffer lengthy detection time at low concentration of analytes because the sensing mechanism relies on stochastic phenomena.In this work, we connected independent membrane sensors in parallel, where the ionic current through the multiple membranes was monitored by a single detector. With this format, the detection time is shortened based on the number of the array and the sensing can be quantitative. We developed a quad sensor assembling four membranes in parallel, and examined detection time of a single membrane sensor, the quad sensor, and four parallel quad-sensors. 1-Gω resistor was installed in series with each membrane to avoid overload current caused by membrane rupture. The results showed significant improvement in the detection time and deviation by the parallelization, which promises quantitative monitoring with the stochastic sensor.

AB - This work designs and demonstrates the lipid bilayer-based ultra-sensitive biosensor capable of short-time detection in a quantitative manner, which is achieved by connecting multiple sensor elements in parallel.Artificial cell membranes with functional membrane proteins can form sensitive/selective biosensors. But the sensors suffer lengthy detection time at low concentration of analytes because the sensing mechanism relies on stochastic phenomena.In this work, we connected independent membrane sensors in parallel, where the ionic current through the multiple membranes was monitored by a single detector. With this format, the detection time is shortened based on the number of the array and the sensing can be quantitative. We developed a quad sensor assembling four membranes in parallel, and examined detection time of a single membrane sensor, the quad sensor, and four parallel quad-sensors. 1-Gω resistor was installed in series with each membrane to avoid overload current caused by membrane rupture. The results showed significant improvement in the detection time and deviation by the parallelization, which promises quantitative monitoring with the stochastic sensor.

KW - Artificial cell membranes

KW - Nanopore

KW - Parallelization

KW - Rapid detection

KW - Stochastic biosensors

UR - http://www.scopus.com/inward/record.url?scp=85071908418&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85071908418&partnerID=8YFLogxK

U2 - 10.1109/TRANSDUCERS.2019.8808191

DO - 10.1109/TRANSDUCERS.2019.8808191

M3 - Conference contribution

AN - SCOPUS:85071908418

T3 - 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII

SP - 6

EP - 8

BT - 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII

PB - Institute of Electrical and Electronics Engineers Inc.

ER -