Quantification of neurotrophins in skeletal muscle following nerve injury

Comparison between spinal cord injury and peripheral nerve injury

Yasushi Morisawa, Shinichiro Takayama, Masaya Nakamura, Toshiyasu Nakamura, Hiroyasu Ikegami, Yoshiaki Toyama

Research output: Contribution to journalArticle

Abstract

Purpose: We investigated the kinematics of nerve growth factor (NGF)mRNA and brain-derived neurotrophic factor (BDNF)mRNA in a skeletal muscle following spinal cord and peripheral nerve injuries by utilizing the reverse-transcription polymerase chain reaction/high-performance liquid chromatography (RT-PCR/HPLC) method. Methods: We made mice models of spinal cord transection and sciatic nerve transection, plus sham and control groups. After RNA extraction from gastrocnemius muscle of mice, RT-PCR was done. We measured the levels of NGFmRNA and BDNFmRNA in a skeletal muscle following spinal cord and peripheral nerve injuries by utilizing the RT-PCR/HPLC method. All values are analyzed and graphed as means ±SED. Bonferroni test (Post-hoc test) was used when two values were compared. Differences were considered statistically significant when p < 0.05. Results: According to the wet muscle weight, at day 7, a significant difference was found between the peripheral lesion and control group, and at days 14 and 28, significant differences were found between the spinal and peripheral lesion and control group. According to the NGFmRNA, in the peripheral nerve injury group, the levels increased up to day 28 and when compared with the control group, the levels were significantly higher at days 14 and 28. According to the BDNFmRNA, levels at days 2, 7, 14, and 28 were significantly lower in the spinal cord injury group than in the control group. However, in peripheral nerve injury group, when compared with the control group, the levels significantly increased at days 7, 14, and 28. Conclusion: We clarified marked differences in chronological changes in neurotrophin kinetics in a skeletal muscle between spinal cord injury and peripheral nerve injury.

Original languageEnglish
Pages (from-to)205-212
Number of pages8
JournalJournal of Musculoskeletal Research
Volume12
Issue number4
DOIs
Publication statusPublished - 2009 Dec

Fingerprint

Peripheral Nerve Injuries
Nerve Growth Factors
Spinal Cord Injuries
Skeletal Muscle
Control Groups
Wounds and Injuries
Spinal Nerves
Polymerase Chain Reaction
Reverse Transcription
Spinal Cord
High Pressure Liquid Chromatography
Messenger RNA
Brain-Derived Neurotrophic Factor
Nerve Growth Factor
Sciatic Nerve
Biomechanical Phenomena
RNA
Weights and Measures
Muscles

Keywords

  • Neurotrophin
  • Peripheral nerve injury
  • RT-PCR/HPLC
  • Skeletal muscle
  • Spinal cord injury

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Quantification of neurotrophins in skeletal muscle following nerve injury : Comparison between spinal cord injury and peripheral nerve injury. / Morisawa, Yasushi; Takayama, Shinichiro; Nakamura, Masaya; Nakamura, Toshiyasu; Ikegami, Hiroyasu; Toyama, Yoshiaki.

In: Journal of Musculoskeletal Research, Vol. 12, No. 4, 12.2009, p. 205-212.

Research output: Contribution to journalArticle

Morisawa, Yasushi ; Takayama, Shinichiro ; Nakamura, Masaya ; Nakamura, Toshiyasu ; Ikegami, Hiroyasu ; Toyama, Yoshiaki. / Quantification of neurotrophins in skeletal muscle following nerve injury : Comparison between spinal cord injury and peripheral nerve injury. In: Journal of Musculoskeletal Research. 2009 ; Vol. 12, No. 4. pp. 205-212.
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