Quantification of plasmodium Falciparum Histidine-rich protein-2 in cerebrospinal spinal fluid from cerebral malaria patients

Kei Mikita, Kiran Thakur, Nicholas M. Anstey, Kim A. Piera, Carlos A. Pardo, J. Brice Weinberg, Jackson Mukemba, Salvatore Florence, Esther D. Mwaikambo, Donald L. Granger, David J. Sullivan

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A cerebrospinal fluid (CSF) biomarker for cerebral malaria (CM) has not been validated. We examined the detection, semiquantification, and clinical use of the Plasmodium falciparum histidine-rich protein-2 (PfHRP-2) as a parasite antigen biomarker for CM. The PfHRP-2 was detected in archival CSF samples from CM patients from Tanzania both by a newly developed sensitive and specific immuno-polymerase chain reaction (72 of 73) and by rapid diagnostic tests (62 of 73). The geometric mean PfHRP-2 CSF concentration was 8.76 ng/mL with no differences in those who survived (9.2 ng/mL), those who died (11.1 ng/mL), and those with neurologic sequelae (10.8 ng/mL). All aparasitemic endemic and nonendemic control samples had undetectable CSF PfHRP-2. In a separate group of 11 matched plasma and CSF cerebral malaria patient samples, the ratio of plasma to CSF PfHRP-2 was 175. The CSF PfHRP-2 reflects elevated plasma PfHRP-2 rather than elevated CM-specific CSF ratios, falling short of a validated biomarker. Copyright

Original languageEnglish
Pages (from-to)486-492
Number of pages7
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume91
Issue number3
DOIs
Publication statusPublished - 2014 Sep 1
Externally publishedYes

Fingerprint

Cerebral Malaria
Plasmodium falciparum
Cerebrospinal Fluid
Biomarkers
Tanzania
histidine-rich proteins
Routine Diagnostic Tests
Nervous System
Parasites
Research Design
Antigens
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

Cite this

Quantification of plasmodium Falciparum Histidine-rich protein-2 in cerebrospinal spinal fluid from cerebral malaria patients. / Mikita, Kei; Thakur, Kiran; Anstey, Nicholas M.; Piera, Kim A.; Pardo, Carlos A.; Weinberg, J. Brice; Mukemba, Jackson; Florence, Salvatore; Mwaikambo, Esther D.; Granger, Donald L.; Sullivan, David J.

In: American Journal of Tropical Medicine and Hygiene, Vol. 91, No. 3, 01.09.2014, p. 486-492.

Research output: Contribution to journalArticle

Mikita, K, Thakur, K, Anstey, NM, Piera, KA, Pardo, CA, Weinberg, JB, Mukemba, J, Florence, S, Mwaikambo, ED, Granger, DL & Sullivan, DJ 2014, 'Quantification of plasmodium Falciparum Histidine-rich protein-2 in cerebrospinal spinal fluid from cerebral malaria patients', American Journal of Tropical Medicine and Hygiene, vol. 91, no. 3, pp. 486-492. https://doi.org/10.4269/ajtmh.14-0210
Mikita, Kei ; Thakur, Kiran ; Anstey, Nicholas M. ; Piera, Kim A. ; Pardo, Carlos A. ; Weinberg, J. Brice ; Mukemba, Jackson ; Florence, Salvatore ; Mwaikambo, Esther D. ; Granger, Donald L. ; Sullivan, David J. / Quantification of plasmodium Falciparum Histidine-rich protein-2 in cerebrospinal spinal fluid from cerebral malaria patients. In: American Journal of Tropical Medicine and Hygiene. 2014 ; Vol. 91, No. 3. pp. 486-492.
@article{26623660d6004dc1882b7d85f6dce60e,
title = "Quantification of plasmodium Falciparum Histidine-rich protein-2 in cerebrospinal spinal fluid from cerebral malaria patients",
abstract = "A cerebrospinal fluid (CSF) biomarker for cerebral malaria (CM) has not been validated. We examined the detection, semiquantification, and clinical use of the Plasmodium falciparum histidine-rich protein-2 (PfHRP-2) as a parasite antigen biomarker for CM. The PfHRP-2 was detected in archival CSF samples from CM patients from Tanzania both by a newly developed sensitive and specific immuno-polymerase chain reaction (72 of 73) and by rapid diagnostic tests (62 of 73). The geometric mean PfHRP-2 CSF concentration was 8.76 ng/mL with no differences in those who survived (9.2 ng/mL), those who died (11.1 ng/mL), and those with neurologic sequelae (10.8 ng/mL). All aparasitemic endemic and nonendemic control samples had undetectable CSF PfHRP-2. In a separate group of 11 matched plasma and CSF cerebral malaria patient samples, the ratio of plasma to CSF PfHRP-2 was 175. The CSF PfHRP-2 reflects elevated plasma PfHRP-2 rather than elevated CM-specific CSF ratios, falling short of a validated biomarker. Copyright",
author = "Kei Mikita and Kiran Thakur and Anstey, {Nicholas M.} and Piera, {Kim A.} and Pardo, {Carlos A.} and Weinberg, {J. Brice} and Jackson Mukemba and Salvatore Florence and Mwaikambo, {Esther D.} and Granger, {Donald L.} and Sullivan, {David J.}",
year = "2014",
month = "9",
day = "1",
doi = "10.4269/ajtmh.14-0210",
language = "English",
volume = "91",
pages = "486--492",
journal = "American Journal of Tropical Medicine and Hygiene",
issn = "0002-9637",
publisher = "American Society of Tropical Medicine and Hygiene",
number = "3",

}

TY - JOUR

T1 - Quantification of plasmodium Falciparum Histidine-rich protein-2 in cerebrospinal spinal fluid from cerebral malaria patients

AU - Mikita, Kei

AU - Thakur, Kiran

AU - Anstey, Nicholas M.

AU - Piera, Kim A.

AU - Pardo, Carlos A.

AU - Weinberg, J. Brice

AU - Mukemba, Jackson

AU - Florence, Salvatore

AU - Mwaikambo, Esther D.

AU - Granger, Donald L.

AU - Sullivan, David J.

PY - 2014/9/1

Y1 - 2014/9/1

N2 - A cerebrospinal fluid (CSF) biomarker for cerebral malaria (CM) has not been validated. We examined the detection, semiquantification, and clinical use of the Plasmodium falciparum histidine-rich protein-2 (PfHRP-2) as a parasite antigen biomarker for CM. The PfHRP-2 was detected in archival CSF samples from CM patients from Tanzania both by a newly developed sensitive and specific immuno-polymerase chain reaction (72 of 73) and by rapid diagnostic tests (62 of 73). The geometric mean PfHRP-2 CSF concentration was 8.76 ng/mL with no differences in those who survived (9.2 ng/mL), those who died (11.1 ng/mL), and those with neurologic sequelae (10.8 ng/mL). All aparasitemic endemic and nonendemic control samples had undetectable CSF PfHRP-2. In a separate group of 11 matched plasma and CSF cerebral malaria patient samples, the ratio of plasma to CSF PfHRP-2 was 175. The CSF PfHRP-2 reflects elevated plasma PfHRP-2 rather than elevated CM-specific CSF ratios, falling short of a validated biomarker. Copyright

AB - A cerebrospinal fluid (CSF) biomarker for cerebral malaria (CM) has not been validated. We examined the detection, semiquantification, and clinical use of the Plasmodium falciparum histidine-rich protein-2 (PfHRP-2) as a parasite antigen biomarker for CM. The PfHRP-2 was detected in archival CSF samples from CM patients from Tanzania both by a newly developed sensitive and specific immuno-polymerase chain reaction (72 of 73) and by rapid diagnostic tests (62 of 73). The geometric mean PfHRP-2 CSF concentration was 8.76 ng/mL with no differences in those who survived (9.2 ng/mL), those who died (11.1 ng/mL), and those with neurologic sequelae (10.8 ng/mL). All aparasitemic endemic and nonendemic control samples had undetectable CSF PfHRP-2. In a separate group of 11 matched plasma and CSF cerebral malaria patient samples, the ratio of plasma to CSF PfHRP-2 was 175. The CSF PfHRP-2 reflects elevated plasma PfHRP-2 rather than elevated CM-specific CSF ratios, falling short of a validated biomarker. Copyright

UR - http://www.scopus.com/inward/record.url?scp=84907016672&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84907016672&partnerID=8YFLogxK

U2 - 10.4269/ajtmh.14-0210

DO - 10.4269/ajtmh.14-0210

M3 - Article

VL - 91

SP - 486

EP - 492

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

SN - 0002-9637

IS - 3

ER -