Quantification of plasmodium Falciparum Histidine-rich protein-2 in cerebrospinal spinal fluid from cerebral malaria patients

Kei Mikita, Kiran Thakur, Nicholas M. Anstey, Kim A. Piera, Carlos A. Pardo, J. Brice Weinberg, Jackson Mukemba, Salvatore Florence, Esther D. Mwaikambo, Donald L. Granger, David J. Sullivan

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A cerebrospinal fluid (CSF) biomarker for cerebral malaria (CM) has not been validated. We examined the detection, semiquantification, and clinical use of the Plasmodium falciparum histidine-rich protein-2 (PfHRP-2) as a parasite antigen biomarker for CM. The PfHRP-2 was detected in archival CSF samples from CM patients from Tanzania both by a newly developed sensitive and specific immuno-polymerase chain reaction (72 of 73) and by rapid diagnostic tests (62 of 73). The geometric mean PfHRP-2 CSF concentration was 8.76 ng/mL with no differences in those who survived (9.2 ng/mL), those who died (11.1 ng/mL), and those with neurologic sequelae (10.8 ng/mL). All aparasitemic endemic and nonendemic control samples had undetectable CSF PfHRP-2. In a separate group of 11 matched plasma and CSF cerebral malaria patient samples, the ratio of plasma to CSF PfHRP-2 was 175. The CSF PfHRP-2 reflects elevated plasma PfHRP-2 rather than elevated CM-specific CSF ratios, falling short of a validated biomarker. Copyright

Original languageEnglish
Pages (from-to)486-492
Number of pages7
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume91
Issue number3
DOIs
Publication statusPublished - 2014 Sep 1

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

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