Quantitation of neurotrophin mRNA in skeletal muscle

Changes during the process of peripheral nerve regeneration

Yasushi Morisawa, Shinichiro Takayama, Kazuhiko Okushi, Toshiyasu Nakamura, Keiichi Fukuda, Yoshihiro Arakawa, Yoshiaki Toyama

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Peripheral nerve injury changes the kinetics of neurotrophins. The production of several neurotrophins increases at the site of injury. Although numerous reports have described changes in neurotrophins over time in areas of nerve injury, neurotrophin mRNA is present at very low levels in target tissues, making accurate quantitation difficult. We developed a reverse transcription-polymerase chain reaction/ high-performance liquid chromatography (RT-PCR/HPLC) method that enables accurate quantitation of neurotrophin mRNA. We then attempted to quantitate mRNA levels for nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) produced by skeletal muscle innervated by the sciatic nerve following transection and reattachment of the nerve in mice. In addition, wet weights of the muscle were measured and changes in weight over time were determined. The results indicated that neurotrophin production in muscle increases as a result of peripheral nerve denervation due to transection, and decreases with nerve regeneration and reinnervation resulting from reattachment.

Original languageEnglish
Pages (from-to)131-140
Number of pages10
JournalJournal of Musculoskeletal Research
Volume10
Issue number3
DOIs
Publication statusPublished - 2006 Sep

Fingerprint

Nerve Regeneration
Nerve Growth Factors
Peripheral Nerves
Skeletal Muscle
Messenger RNA
Weights and Measures
Peripheral Nerve Injuries
Muscles
Brain-Derived Neurotrophic Factor
Wounds and Injuries
Nerve Growth Factor
Denervation
Sciatic Nerve
Reverse Transcription
High Pressure Liquid Chromatography
Polymerase Chain Reaction

Keywords

  • Neurotrophin
  • Peripheral nerve
  • RT-PCR/HPLC method
  • Skeletal muscle

ASJC Scopus subject areas

  • Surgery

Cite this

Quantitation of neurotrophin mRNA in skeletal muscle : Changes during the process of peripheral nerve regeneration. / Morisawa, Yasushi; Takayama, Shinichiro; Okushi, Kazuhiko; Nakamura, Toshiyasu; Fukuda, Keiichi; Arakawa, Yoshihiro; Toyama, Yoshiaki.

In: Journal of Musculoskeletal Research, Vol. 10, No. 3, 09.2006, p. 131-140.

Research output: Contribution to journalArticle

Morisawa, Yasushi ; Takayama, Shinichiro ; Okushi, Kazuhiko ; Nakamura, Toshiyasu ; Fukuda, Keiichi ; Arakawa, Yoshihiro ; Toyama, Yoshiaki. / Quantitation of neurotrophin mRNA in skeletal muscle : Changes during the process of peripheral nerve regeneration. In: Journal of Musculoskeletal Research. 2006 ; Vol. 10, No. 3. pp. 131-140.
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