Quantitative assessment of tendon healing by using MR T2 mapping in a rabbit achilles tendon transection model treated with platelet-rich plasma

Taisuke Fukawa, Satoshi Yamaguchi, Atsuya Watanabe, Takahisa Sasho, Ryuichiro Akagi, Yuta Muramatsu, Yorikazu Akatsu, Joe Katsuragi, Jun Endo, Fumio Osone, Yasunori Sato, Toshiyuki Okubo, Kazuhisa Takahashi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Purpose: To determine if magnetic resonance (MR) imaging T2 mapping can be used to quantify histologic tendon healing by using a rabbit Achilles tendon transection model treated with platelet-rich plasma (PRP). Materials and Methods: Experiments were approved by the Institutional Animal Care and Use Committee. The Achilles tendons of 24 New Zealand white rabbits (48 limbs) were surgically transected, and PRP (in the test group) or saline (in the control group) was injected into the transection site. The rabbits were sacrificed 2, 4, 8, and 12 weeks after surgery. Thereafter, T2 mapping and histologic evaluations were performed by using the Bonar scale. A mixed-model multivariate analysis of variance was used to test the effects of time and PRP treatment on the T2 value and Bonar grade, respectively. The correlation between the T2 value and Bonar grade was also assessed by using the Spearman correlation coefficient. Results: The Bonar scale values decreased in both groups during tendon healing. The T2 value also shortened over time (P < .001 for both groups). The T2 values were positively correlated with the Bonar grade (r = 0.78, P < .001). Both the T2 value and Bonar scale value were lower in the PRP group than in the control group at 4, 8, and 12 weeks; however, there was no significant effect of PRP treatment on the T2 value or Bonar grade. Conclusion: The T2 value changes reflected histologic tendon healing. While T2 and Bonar grade were lower at all time points in tendons treated with PRP, there was no significant difference between the treatment and control tendons.

Original languageEnglish
Pages (from-to)748-755
Number of pages8
JournalRadiology
Volume276
Issue number3
DOIs
Publication statusPublished - 2015 Sep 1
Externally publishedYes

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Achilles Tendon
Platelet-Rich Plasma
Tendons
Magnetic Resonance Spectroscopy
Rabbits
Animal Care Committees
Control Groups
Analysis of Variance
Multivariate Analysis
Extremities
Magnetic Resonance Imaging

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Quantitative assessment of tendon healing by using MR T2 mapping in a rabbit achilles tendon transection model treated with platelet-rich plasma. / Fukawa, Taisuke; Yamaguchi, Satoshi; Watanabe, Atsuya; Sasho, Takahisa; Akagi, Ryuichiro; Muramatsu, Yuta; Akatsu, Yorikazu; Katsuragi, Joe; Endo, Jun; Osone, Fumio; Sato, Yasunori; Okubo, Toshiyuki; Takahashi, Kazuhisa.

In: Radiology, Vol. 276, No. 3, 01.09.2015, p. 748-755.

Research output: Contribution to journalArticle

Fukawa, T, Yamaguchi, S, Watanabe, A, Sasho, T, Akagi, R, Muramatsu, Y, Akatsu, Y, Katsuragi, J, Endo, J, Osone, F, Sato, Y, Okubo, T & Takahashi, K 2015, 'Quantitative assessment of tendon healing by using MR T2 mapping in a rabbit achilles tendon transection model treated with platelet-rich plasma', Radiology, vol. 276, no. 3, pp. 748-755. https://doi.org/10.1148/radiol.2015141544
Fukawa, Taisuke ; Yamaguchi, Satoshi ; Watanabe, Atsuya ; Sasho, Takahisa ; Akagi, Ryuichiro ; Muramatsu, Yuta ; Akatsu, Yorikazu ; Katsuragi, Joe ; Endo, Jun ; Osone, Fumio ; Sato, Yasunori ; Okubo, Toshiyuki ; Takahashi, Kazuhisa. / Quantitative assessment of tendon healing by using MR T2 mapping in a rabbit achilles tendon transection model treated with platelet-rich plasma. In: Radiology. 2015 ; Vol. 276, No. 3. pp. 748-755.
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abstract = "Purpose: To determine if magnetic resonance (MR) imaging T2 mapping can be used to quantify histologic tendon healing by using a rabbit Achilles tendon transection model treated with platelet-rich plasma (PRP). Materials and Methods: Experiments were approved by the Institutional Animal Care and Use Committee. The Achilles tendons of 24 New Zealand white rabbits (48 limbs) were surgically transected, and PRP (in the test group) or saline (in the control group) was injected into the transection site. The rabbits were sacrificed 2, 4, 8, and 12 weeks after surgery. Thereafter, T2 mapping and histologic evaluations were performed by using the Bonar scale. A mixed-model multivariate analysis of variance was used to test the effects of time and PRP treatment on the T2 value and Bonar grade, respectively. The correlation between the T2 value and Bonar grade was also assessed by using the Spearman correlation coefficient. Results: The Bonar scale values decreased in both groups during tendon healing. The T2 value also shortened over time (P < .001 for both groups). The T2 values were positively correlated with the Bonar grade (r = 0.78, P < .001). Both the T2 value and Bonar scale value were lower in the PRP group than in the control group at 4, 8, and 12 weeks; however, there was no significant effect of PRP treatment on the T2 value or Bonar grade. Conclusion: The T2 value changes reflected histologic tendon healing. While T2 and Bonar grade were lower at all time points in tendons treated with PRP, there was no significant difference between the treatment and control tendons.",
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AU - Watanabe, Atsuya

AU - Sasho, Takahisa

AU - Akagi, Ryuichiro

AU - Muramatsu, Yuta

AU - Akatsu, Yorikazu

AU - Katsuragi, Joe

AU - Endo, Jun

AU - Osone, Fumio

AU - Sato, Yasunori

AU - Okubo, Toshiyuki

AU - Takahashi, Kazuhisa

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N2 - Purpose: To determine if magnetic resonance (MR) imaging T2 mapping can be used to quantify histologic tendon healing by using a rabbit Achilles tendon transection model treated with platelet-rich plasma (PRP). Materials and Methods: Experiments were approved by the Institutional Animal Care and Use Committee. The Achilles tendons of 24 New Zealand white rabbits (48 limbs) were surgically transected, and PRP (in the test group) or saline (in the control group) was injected into the transection site. The rabbits were sacrificed 2, 4, 8, and 12 weeks after surgery. Thereafter, T2 mapping and histologic evaluations were performed by using the Bonar scale. A mixed-model multivariate analysis of variance was used to test the effects of time and PRP treatment on the T2 value and Bonar grade, respectively. The correlation between the T2 value and Bonar grade was also assessed by using the Spearman correlation coefficient. Results: The Bonar scale values decreased in both groups during tendon healing. The T2 value also shortened over time (P < .001 for both groups). The T2 values were positively correlated with the Bonar grade (r = 0.78, P < .001). Both the T2 value and Bonar scale value were lower in the PRP group than in the control group at 4, 8, and 12 weeks; however, there was no significant effect of PRP treatment on the T2 value or Bonar grade. Conclusion: The T2 value changes reflected histologic tendon healing. While T2 and Bonar grade were lower at all time points in tendons treated with PRP, there was no significant difference between the treatment and control tendons.

AB - Purpose: To determine if magnetic resonance (MR) imaging T2 mapping can be used to quantify histologic tendon healing by using a rabbit Achilles tendon transection model treated with platelet-rich plasma (PRP). Materials and Methods: Experiments were approved by the Institutional Animal Care and Use Committee. The Achilles tendons of 24 New Zealand white rabbits (48 limbs) were surgically transected, and PRP (in the test group) or saline (in the control group) was injected into the transection site. The rabbits were sacrificed 2, 4, 8, and 12 weeks after surgery. Thereafter, T2 mapping and histologic evaluations were performed by using the Bonar scale. A mixed-model multivariate analysis of variance was used to test the effects of time and PRP treatment on the T2 value and Bonar grade, respectively. The correlation between the T2 value and Bonar grade was also assessed by using the Spearman correlation coefficient. Results: The Bonar scale values decreased in both groups during tendon healing. The T2 value also shortened over time (P < .001 for both groups). The T2 values were positively correlated with the Bonar grade (r = 0.78, P < .001). Both the T2 value and Bonar scale value were lower in the PRP group than in the control group at 4, 8, and 12 weeks; however, there was no significant effect of PRP treatment on the T2 value or Bonar grade. Conclusion: The T2 value changes reflected histologic tendon healing. While T2 and Bonar grade were lower at all time points in tendons treated with PRP, there was no significant difference between the treatment and control tendons.

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