Quantitative Detection of Target ssDNA by digitally counting gold nanoparticle Dimers

Takaha Mizuguchi, Keiko Esashika, Toshiharu Saiki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We presented a quantitative detection of target single-stranded DNA (ssDNA) by digitally counting gold nanoparticle (AuNP) dimers in AuNP suspension using a confocal optical setup. The AuNP dimers were formed by hybridization between two kinds of ssDNA probes immobilized on the AuNP surface and a complementary ssDNA target. The AuNPs were also modified with a self-Assembled monolayer for good dispersibility in a suspension. AuNP monomers and dimers could be distinguished simply by the scattered light intensity from AuNPs at a detection limit of sub-10 pM level.

Original languageEnglish
Title of host publicationOptics and Photonics Japan, OFJ 2017
PublisherOptica Publishing Group (formerly OSA)
ISBN (Electronic)9781557528209
Publication statusPublished - 2017
EventOptics and Photonics Japan, OFJ 2017 - Tokyo, Japan
Duration: 2017 Oct 302017 Oct 31

Publication series

NameOptics InfoBase Conference Papers

Conference

ConferenceOptics and Photonics Japan, OFJ 2017
Country/TerritoryJapan
CityTokyo
Period17/10/3017/10/31

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Mechanics of Materials

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