Abstract
Enantiomers of disopyramide (DP), flecainide (FLC) and verapamil (VP) were extracted from rat plasma and tissues (brain, lung, heart, liver, kidney and muscle), followed by quantitative determination using enantioselective highperformance liquid chromatography with chiral stationary-phase columns. The recoveries of S-(+)- and R-(-)-DP from tissues were higher than 69%, and the within- and between-day coefficients of variation were very low (0.5 - 5.7%). The lower limits of detection in each tissue were less than 289 ng/g tissue. The recoveries of S-(+)- and R-(-)-FLC from tissues were higher than 88%, and the within- and between-day coefficients of variation were 1.2- 6.0%. The lower limits of detection in each tissue were less than 37 ng/g tissue. The recoveries of S-(-)- and R-(+)-VP from tissues were higher than 80%, and the within- and between-day coefficients of variation were 0.5- 6.2%. The lower limits of detection in each tissue were less than 51 ng/g tissue. The analytical methods established in this study will be suitable for determining the concentrations of the enantiomers of these anti-arrhythmic agents in rat plasma and tissues.
| Original language | English |
|---|---|
| Pages (from-to) | 129-135 |
| Number of pages | 7 |
| Journal | Journal of Chromatography B: Biomedical Applications |
| Volume | 710 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 1998 Jun 12 |
| Externally published | Yes |
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Keywords
- Disopyramide
- Enantiomer separation
- Flecainide
- Verapamil
ASJC Scopus subject areas
- Chemistry(all)
Cite this
Quantitative determination of disopyramide, verapamil and flecainide enantiomers in rat plasma and tissues by high-performance liquid chromatography. / Hanada, Kazuhiko; Akimoto, Satoko; Mitsui, Keiko; Hashiguchi, Masayuki; Ogata, Hiroyasu.
In: Journal of Chromatography B: Biomedical Applications, Vol. 710, No. 1-2, 12.06.1998, p. 129-135.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quantitative determination of disopyramide, verapamil and flecainide enantiomers in rat plasma and tissues by high-performance liquid chromatography
AU - Hanada, Kazuhiko
AU - Akimoto, Satoko
AU - Mitsui, Keiko
AU - Hashiguchi, Masayuki
AU - Ogata, Hiroyasu
PY - 1998/6/12
Y1 - 1998/6/12
N2 - Enantiomers of disopyramide (DP), flecainide (FLC) and verapamil (VP) were extracted from rat plasma and tissues (brain, lung, heart, liver, kidney and muscle), followed by quantitative determination using enantioselective highperformance liquid chromatography with chiral stationary-phase columns. The recoveries of S-(+)- and R-(-)-DP from tissues were higher than 69%, and the within- and between-day coefficients of variation were very low (0.5 - 5.7%). The lower limits of detection in each tissue were less than 289 ng/g tissue. The recoveries of S-(+)- and R-(-)-FLC from tissues were higher than 88%, and the within- and between-day coefficients of variation were 1.2- 6.0%. The lower limits of detection in each tissue were less than 37 ng/g tissue. The recoveries of S-(-)- and R-(+)-VP from tissues were higher than 80%, and the within- and between-day coefficients of variation were 0.5- 6.2%. The lower limits of detection in each tissue were less than 51 ng/g tissue. The analytical methods established in this study will be suitable for determining the concentrations of the enantiomers of these anti-arrhythmic agents in rat plasma and tissues.
AB - Enantiomers of disopyramide (DP), flecainide (FLC) and verapamil (VP) were extracted from rat plasma and tissues (brain, lung, heart, liver, kidney and muscle), followed by quantitative determination using enantioselective highperformance liquid chromatography with chiral stationary-phase columns. The recoveries of S-(+)- and R-(-)-DP from tissues were higher than 69%, and the within- and between-day coefficients of variation were very low (0.5 - 5.7%). The lower limits of detection in each tissue were less than 289 ng/g tissue. The recoveries of S-(+)- and R-(-)-FLC from tissues were higher than 88%, and the within- and between-day coefficients of variation were 1.2- 6.0%. The lower limits of detection in each tissue were less than 37 ng/g tissue. The recoveries of S-(-)- and R-(+)-VP from tissues were higher than 80%, and the within- and between-day coefficients of variation were 0.5- 6.2%. The lower limits of detection in each tissue were less than 51 ng/g tissue. The analytical methods established in this study will be suitable for determining the concentrations of the enantiomers of these anti-arrhythmic agents in rat plasma and tissues.
KW - Disopyramide
KW - Enantiomer separation
KW - Flecainide
KW - Verapamil
UR - http://www.scopus.com/inward/record.url?scp=0032511239&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032511239&partnerID=8YFLogxK
U2 - 10.1016/S0378-4347(98)00112-1
DO - 10.1016/S0378-4347(98)00112-1
M3 - Article
VL - 710
SP - 129
EP - 135
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
SN - 0378-4347
IS - 1-2
ER -