Quantitative determination of disopyramide, verapamil and flecainide enantiomers in rat plasma and tissues by high-performance liquid chromatography

Kazuhiko Hanada, Satoko Akimoto, Keiko Mitsui, Masayuki Hashiguchi, Hiroyasu Ogata

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Enantiomers of disopyramide (DP), flecainide (FLC) and verapamil (VP) were extracted from rat plasma and tissues (brain, lung, heart, liver, kidney and muscle), followed by quantitative determination using enantioselective highperformance liquid chromatography with chiral stationary-phase columns. The recoveries of S-(+)- and R-(-)-DP from tissues were higher than 69%, and the within- and between-day coefficients of variation were very low (0.5 - 5.7%). The lower limits of detection in each tissue were less than 289 ng/g tissue. The recoveries of S-(+)- and R-(-)-FLC from tissues were higher than 88%, and the within- and between-day coefficients of variation were 1.2- 6.0%. The lower limits of detection in each tissue were less than 37 ng/g tissue. The recoveries of S-(-)- and R-(+)-VP from tissues were higher than 80%, and the within- and between-day coefficients of variation were 0.5- 6.2%. The lower limits of detection in each tissue were less than 51 ng/g tissue. The analytical methods established in this study will be suitable for determining the concentrations of the enantiomers of these anti-arrhythmic agents in rat plasma and tissues.

Original languageEnglish
Pages (from-to)129-135
Number of pages7
JournalJournal of Chromatography B: Biomedical Applications
Volume710
Issue number1-2
DOIs
Publication statusPublished - 1998 Jun 12
Externally publishedYes

Keywords

  • Disopyramide
  • Enantiomer separation
  • Flecainide
  • Verapamil

ASJC Scopus subject areas

  • Chemistry(all)

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