Quantitative determination of epinastine in plasma by high-performance liquid chromatography

Hisakazu Ohtani, Hajime Kotaki, Yasufumi Sawada, Tatsuji Iga

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A high-performance liquid chromatographic (HPLC) method for the quantitative determination of epinastine, a non-sedating histamine H1 receptor antagonist, in rat plasma, was developed. A 100-μl volume of plasma sample was spiked with a solution of internal standard (diphenidol) and extracted with dichloromethane under alkaline conditions. The extract was applied onto the HPLC system and detected by ultraviolet absorption at a wavelength of 220 nm. The linearity of the calibration curve was preserved over the concentration range of 20-1000 ng/ml. Both intra-assay variation and relative error were less than 5% for the plasma sample containing 50 ng/ml or 1000 ng/ml of epinastine hydrochloride. The analytical method presented here should be useful for the investigation of the pharmacokinetic properties of epinastine, which is of clinical significance.

Original languageEnglish
Pages (from-to)281-284
Number of pages4
JournalJournal of Chromatography B: Biomedical Applications
Volume683
Issue number2
DOIs
Publication statusPublished - 1996 Aug 30
Externally publishedYes

Fingerprint

High performance liquid chromatography
Plasmas
Histamine H1 Antagonists
Pharmacokinetics
Methylene Chloride
Liquids
Rats
Assays
Calibration
Wavelength
epinastine

Keywords

  • Epinastine

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Quantitative determination of epinastine in plasma by high-performance liquid chromatography. / Ohtani, Hisakazu; Kotaki, Hajime; Sawada, Yasufumi; Iga, Tatsuji.

In: Journal of Chromatography B: Biomedical Applications, Vol. 683, No. 2, 30.08.1996, p. 281-284.

Research output: Contribution to journalArticle

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