Quantitative measurements of the interaction between monosialoganglioside monolayers and wheat germ agglutinin (WGA) by a quartz- crystal microbalance

Toshinori Sato, Takeshi Serizawa, Fuyuka Ohtake, Miwa Nakamura, Takashi Terabayashi, Yasuhiro Kawanishi, Yoshio Okahata

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Monosialogangliosides (GM1, GM2, GM3 and GM4) were reconstituted in lipid monolayers at the air-water interface. The binding amounts and the initial binding rates of wheat germ agglutinin (WGA) to the monosialoganglioside monolayers were quantitatively studied by use of a quartz-crystal microbalance (QCM). A QCM was horizontally attached to the monolayer from the air phase, and the binding behavior (mass increase) was followed by the frequency decrease of the QCM. WGA binding affinities for the ganglioside monolayers were influenced by hydrophilic head groups of lipid matrices, densities of gangliosides, and sequences of oligosaccharide in gangliosides. Binding of WGA to the gangliosides reconstituted in a phosphatidylcholine (sphingomyelin and distearoylphosphatidylcholine) matrix was strongly suppressed, but not in a neutral glycolipids (GlcCer, GalCer, and LacCer), dipalmitoylphosphatidylethanolamine, and dipalmitoylphosphatidylethanolamine matrix. WGA showed high affinity for monolayers contaning 20 mol% gangliosides, but only low affinity for 100% ganglioside monolayers. WGA preferably binds to gangliosides in the following sequence: GM3 > GM4 > > GM2 = GM1. No affinities of WGA for GM2 and GM1 were observed. The combined techniques of monolayer and QCM have the advantages of investigating recognition properties of gangliosides.

Original languageEnglish
Pages (from-to)82-92
Number of pages11
JournalBiochimica et Biophysica Acta - General Subjects
Volume1380
Issue number1
DOIs
Publication statusPublished - 1998 Mar 12
Externally publishedYes

Fingerprint

Quartz Crystal Microbalance Techniques
Wheat Germ Agglutinins
Gangliosides
Quartz crystal microbalances
Monolayers
Mass Behavior
Air
Lipids
Sphingomyelins
sialogangliosides
Glycolipids
Phosphatidylcholines
Oligosaccharides
Head
Water

Keywords

  • Monosialoganglioside
  • Wheat germ agglutinin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Quantitative measurements of the interaction between monosialoganglioside monolayers and wheat germ agglutinin (WGA) by a quartz- crystal microbalance. / Sato, Toshinori; Serizawa, Takeshi; Ohtake, Fuyuka; Nakamura, Miwa; Terabayashi, Takashi; Kawanishi, Yasuhiro; Okahata, Yoshio.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 1380, No. 1, 12.03.1998, p. 82-92.

Research output: Contribution to journalArticle

Sato, Toshinori ; Serizawa, Takeshi ; Ohtake, Fuyuka ; Nakamura, Miwa ; Terabayashi, Takashi ; Kawanishi, Yasuhiro ; Okahata, Yoshio. / Quantitative measurements of the interaction between monosialoganglioside monolayers and wheat germ agglutinin (WGA) by a quartz- crystal microbalance. In: Biochimica et Biophysica Acta - General Subjects. 1998 ; Vol. 1380, No. 1. pp. 82-92.
@article{6c6da086bdb2483d9bf587cc1ebd1a11,
title = "Quantitative measurements of the interaction between monosialoganglioside monolayers and wheat germ agglutinin (WGA) by a quartz- crystal microbalance",
abstract = "Monosialogangliosides (GM1, GM2, GM3 and GM4) were reconstituted in lipid monolayers at the air-water interface. The binding amounts and the initial binding rates of wheat germ agglutinin (WGA) to the monosialoganglioside monolayers were quantitatively studied by use of a quartz-crystal microbalance (QCM). A QCM was horizontally attached to the monolayer from the air phase, and the binding behavior (mass increase) was followed by the frequency decrease of the QCM. WGA binding affinities for the ganglioside monolayers were influenced by hydrophilic head groups of lipid matrices, densities of gangliosides, and sequences of oligosaccharide in gangliosides. Binding of WGA to the gangliosides reconstituted in a phosphatidylcholine (sphingomyelin and distearoylphosphatidylcholine) matrix was strongly suppressed, but not in a neutral glycolipids (GlcCer, GalCer, and LacCer), dipalmitoylphosphatidylethanolamine, and dipalmitoylphosphatidylethanolamine matrix. WGA showed high affinity for monolayers contaning 20 mol{\%} gangliosides, but only low affinity for 100{\%} ganglioside monolayers. WGA preferably binds to gangliosides in the following sequence: GM3 > GM4 > > GM2 = GM1. No affinities of WGA for GM2 and GM1 were observed. The combined techniques of monolayer and QCM have the advantages of investigating recognition properties of gangliosides.",
keywords = "Monosialoganglioside, Wheat germ agglutinin",
author = "Toshinori Sato and Takeshi Serizawa and Fuyuka Ohtake and Miwa Nakamura and Takashi Terabayashi and Yasuhiro Kawanishi and Yoshio Okahata",
year = "1998",
month = "3",
day = "12",
doi = "10.1016/S0304-4165(97)00133-5",
language = "English",
volume = "1380",
pages = "82--92",
journal = "Biochimica et Biophysica Acta - General Subjects",
issn = "0006-3002",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Quantitative measurements of the interaction between monosialoganglioside monolayers and wheat germ agglutinin (WGA) by a quartz- crystal microbalance

AU - Sato, Toshinori

AU - Serizawa, Takeshi

AU - Ohtake, Fuyuka

AU - Nakamura, Miwa

AU - Terabayashi, Takashi

AU - Kawanishi, Yasuhiro

AU - Okahata, Yoshio

PY - 1998/3/12

Y1 - 1998/3/12

N2 - Monosialogangliosides (GM1, GM2, GM3 and GM4) were reconstituted in lipid monolayers at the air-water interface. The binding amounts and the initial binding rates of wheat germ agglutinin (WGA) to the monosialoganglioside monolayers were quantitatively studied by use of a quartz-crystal microbalance (QCM). A QCM was horizontally attached to the monolayer from the air phase, and the binding behavior (mass increase) was followed by the frequency decrease of the QCM. WGA binding affinities for the ganglioside monolayers were influenced by hydrophilic head groups of lipid matrices, densities of gangliosides, and sequences of oligosaccharide in gangliosides. Binding of WGA to the gangliosides reconstituted in a phosphatidylcholine (sphingomyelin and distearoylphosphatidylcholine) matrix was strongly suppressed, but not in a neutral glycolipids (GlcCer, GalCer, and LacCer), dipalmitoylphosphatidylethanolamine, and dipalmitoylphosphatidylethanolamine matrix. WGA showed high affinity for monolayers contaning 20 mol% gangliosides, but only low affinity for 100% ganglioside monolayers. WGA preferably binds to gangliosides in the following sequence: GM3 > GM4 > > GM2 = GM1. No affinities of WGA for GM2 and GM1 were observed. The combined techniques of monolayer and QCM have the advantages of investigating recognition properties of gangliosides.

AB - Monosialogangliosides (GM1, GM2, GM3 and GM4) were reconstituted in lipid monolayers at the air-water interface. The binding amounts and the initial binding rates of wheat germ agglutinin (WGA) to the monosialoganglioside monolayers were quantitatively studied by use of a quartz-crystal microbalance (QCM). A QCM was horizontally attached to the monolayer from the air phase, and the binding behavior (mass increase) was followed by the frequency decrease of the QCM. WGA binding affinities for the ganglioside monolayers were influenced by hydrophilic head groups of lipid matrices, densities of gangliosides, and sequences of oligosaccharide in gangliosides. Binding of WGA to the gangliosides reconstituted in a phosphatidylcholine (sphingomyelin and distearoylphosphatidylcholine) matrix was strongly suppressed, but not in a neutral glycolipids (GlcCer, GalCer, and LacCer), dipalmitoylphosphatidylethanolamine, and dipalmitoylphosphatidylethanolamine matrix. WGA showed high affinity for monolayers contaning 20 mol% gangliosides, but only low affinity for 100% ganglioside monolayers. WGA preferably binds to gangliosides in the following sequence: GM3 > GM4 > > GM2 = GM1. No affinities of WGA for GM2 and GM1 were observed. The combined techniques of monolayer and QCM have the advantages of investigating recognition properties of gangliosides.

KW - Monosialoganglioside

KW - Wheat germ agglutinin

UR - http://www.scopus.com/inward/record.url?scp=0032510284&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032510284&partnerID=8YFLogxK

U2 - 10.1016/S0304-4165(97)00133-5

DO - 10.1016/S0304-4165(97)00133-5

M3 - Article

VL - 1380

SP - 82

EP - 92

JO - Biochimica et Biophysica Acta - General Subjects

JF - Biochimica et Biophysica Acta - General Subjects

SN - 0006-3002

IS - 1

ER -