Quantitative Metabolome Analysis Using Capillary Electrophoresis Mass Spectrometry

Tomoyoshi Soga, Yoshiaki Ohashi, Yuki Ueno, Hisako Naraoka, Masaru Tomita, Takaaki Nishioka

Research output: Contribution to journalArticle

671 Citations (Scopus)

Abstract

A new approach for the comprehensive and quantitative analysis of charged metabolites by capillary electrophoresis mass spectrometry (CE-MS) is proposed. Metabolites are first separated by CE based on charge and size and then selectively detected using MS by monitoring over a large range of m/z values. This method enabled the determination of 352 metabolic standards and its utility was demonstrated in the analysis of 1692 metabolites from Bacillus subtilis extracts, revealing significant changes in metabolites during B. subtilis sporulation.

Original languageEnglish
Pages (from-to)488-494
Number of pages7
JournalJournal of Proteome Research
Volume2
Issue number5
DOIs
Publication statusPublished - 2003 Sep

Fingerprint

Capillary electrophoresis
Metabolome
Capillary Electrophoresis
Metabolites
Bacillus subtilis
Mass spectrometry
Mass Spectrometry
Chemical analysis
Bacilli
Monitoring

Keywords

  • Bacillus subtilis
  • Capillary electrophoresis
  • CoA
  • Glycolysis
  • Mass spectrometry
  • Metabolic pathways
  • Metabolomics
  • Nucleotide
  • Sporulation
  • TCA

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Biochemistry

Cite this

Quantitative Metabolome Analysis Using Capillary Electrophoresis Mass Spectrometry. / Soga, Tomoyoshi; Ohashi, Yoshiaki; Ueno, Yuki; Naraoka, Hisako; Tomita, Masaru; Nishioka, Takaaki.

In: Journal of Proteome Research, Vol. 2, No. 5, 09.2003, p. 488-494.

Research output: Contribution to journalArticle

Soga, Tomoyoshi ; Ohashi, Yoshiaki ; Ueno, Yuki ; Naraoka, Hisako ; Tomita, Masaru ; Nishioka, Takaaki. / Quantitative Metabolome Analysis Using Capillary Electrophoresis Mass Spectrometry. In: Journal of Proteome Research. 2003 ; Vol. 2, No. 5. pp. 488-494.
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