Quaternary structure of LOV-domain containing polypeptide of Arabidopsis FKF1 protein

Masayoshi Nakasako, Daisuke Matsuoka, Kazunori Zikihara, Satoru Tokutomi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Flavin-binding, Kelch repeat, F-box (FKF1) protein is a photoreceptor to regulate flowering of Arabidopsis. The protein has a light, oxygen and voltage (LOV)-sensing domain binding a flavin mononucleotide. The photo-activation of the domain is an indispensable step to initiate the cellular signaling for flowering. In the present study, a LOV-containing polypeptide of FKF1 was prepared by an overexpression system, and the quaternary structure of it was studied by size exclusion chromatography and small-angle X-ray scattering. The apparent molecular weight from chromatography suggested a globular trimeric or an anisotropic-shaped dimeric association of the polypeptide in solution. The scattering experiment demonstrated a dimeric association of the polypeptides with an elongated molecular shape displaying the radius of gyration of 27 Å and the maximum dimension of 94 Å. The molecular shape simulated from scattering profiles suggests an antiparallel association of the LOV domains in the dimer. Though the absorption spectrum of blue-light irradiated polypeptide was stable in the photoactivated state for a long period, the scattering profiles showed very small changes between the dark and light conditions. Based on the homologies in the amino-acid sequences and the scattering profiles, these results are discussed in connection with the structures and function of LOV domains of phototropin.

Original languageEnglish
Pages (from-to)1067-1071
Number of pages5
JournalFEBS Letters
Volume579
Issue number5
DOIs
Publication statusPublished - 2005 Feb 14

Fingerprint

Oxygen
Light
Peptides
Electric potential
Scattering
Association reactions
Phototropins
F-Box Proteins
Cell signaling
Flavin Mononucleotide
Amino Acid Sequence Homology
Size exclusion chromatography
Chromatography
X ray scattering
Arabidopsis
Dimers
Gel Chromatography
Arabidopsis FKF1 protein
Absorption spectra
Proteins

Keywords

  • FKF1 protein
  • LOV domain
  • Photoperiodism
  • Quaternary structure
  • Small-angle X-ray scattering

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Quaternary structure of LOV-domain containing polypeptide of Arabidopsis FKF1 protein. / Nakasako, Masayoshi; Matsuoka, Daisuke; Zikihara, Kazunori; Tokutomi, Satoru.

In: FEBS Letters, Vol. 579, No. 5, 14.02.2005, p. 1067-1071.

Research output: Contribution to journalArticle

Nakasako, Masayoshi ; Matsuoka, Daisuke ; Zikihara, Kazunori ; Tokutomi, Satoru. / Quaternary structure of LOV-domain containing polypeptide of Arabidopsis FKF1 protein. In: FEBS Letters. 2005 ; Vol. 579, No. 5. pp. 1067-1071.
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