Quick-freeze, deep-etch visualization of the nuclear pore complex

Youhei Fujitani; Shouzou Higaki; Hajime Sawada; Kazushige Hirosawa

Quick-freeze, deep-etch visualization of the nuclear pore complex

Youhei Fujitani, Shouzou Higaki, Hajime Sawada, Kazushige Hirosawa

Research output: Contribution to journal › Article › peer-review

Abstract

We have visualized the nuclear pore complex (NPCx) three-dimensionally by quick-freeze, deep etching and suggest that NPCx is composed of four rings and associated filaments. The rings were located at the cytoplasmic side, at the nucleoplasmic side, and at the pore waist as well. Some of the filaments emanated toward the cytoplasm, some sprawled over the cytoplasmic face of the nuclear membrane, and some intervened the rings. The emanating filaments were considered to be vimentin filaments judging from the replica and thin-sectioned image.

Original language	English
Pages (from-to)	34-40
Number of pages	7
Journal	Journal of Electron Microscopy
Volume	38
Issue number	1
Publication status	Published - 1989
Externally published	Yes

Keywords

Deep-etching
Intermediate filament
Nuclear membrane
Nuclear pore complex
Osteosarcoma
Quick-freeze

ASJC Scopus subject areas

Instrumentation

Cite this

@article{e0db422d8d054600a7f43725bb0e1f49,

title = "Quick-freeze, deep-etch visualization of the nuclear pore complex",

abstract = "We have visualized the nuclear pore complex (NPCx) three-dimensionally by quick-freeze, deep etching and suggest that NPCx is composed of four rings and associated filaments. The rings were located at the cytoplasmic side, at the nucleoplasmic side, and at the pore waist as well. Some of the filaments emanated toward the cytoplasm, some sprawled over the cytoplasmic face of the nuclear membrane, and some intervened the rings. The emanating filaments were considered to be vimentin filaments judging from the replica and thin-sectioned image.",

keywords = "Deep-etching, Intermediate filament, Nuclear membrane, Nuclear pore complex, Osteosarcoma, Quick-freeze",

author = "Youhei Fujitani and Shouzou Higaki and Hajime Sawada and Kazushige Hirosawa",

note = "Funding Information: Acknowledgments. We would like to thank Dr. A. Matsuzawa, Laboratory Animal Research Center, Institute of Medical Science, and Dr. R. Toyama, Department of Chemistry, Institute of Medical Science, for helpful advice. We would like to thank Ms. M. Inoue for typewriting, and also thank Ms. C. Ishiguro for advice on some English phrases. This work was supported by a Grant-in-Aid for Cancer Research (K. H. 61010024) from the Ministry of Education, Science and Culture, Japan.",

year = "1989",

language = "English",

volume = "38",

pages = "34--40",

journal = "Microscopy (Oxford, England)",

issn = "2050-5698",

publisher = "Japanese Society of Microscopy",

number = "1",

}

TY - JOUR

T1 - Quick-freeze, deep-etch visualization of the nuclear pore complex

AU - Fujitani, Youhei

AU - Higaki, Shouzou

AU - Sawada, Hajime

AU - Hirosawa, Kazushige

N1 - Funding Information: Acknowledgments. We would like to thank Dr. A. Matsuzawa, Laboratory Animal Research Center, Institute of Medical Science, and Dr. R. Toyama, Department of Chemistry, Institute of Medical Science, for helpful advice. We would like to thank Ms. M. Inoue for typewriting, and also thank Ms. C. Ishiguro for advice on some English phrases. This work was supported by a Grant-in-Aid for Cancer Research (K. H. 61010024) from the Ministry of Education, Science and Culture, Japan.

PY - 1989

Y1 - 1989

N2 - We have visualized the nuclear pore complex (NPCx) three-dimensionally by quick-freeze, deep etching and suggest that NPCx is composed of four rings and associated filaments. The rings were located at the cytoplasmic side, at the nucleoplasmic side, and at the pore waist as well. Some of the filaments emanated toward the cytoplasm, some sprawled over the cytoplasmic face of the nuclear membrane, and some intervened the rings. The emanating filaments were considered to be vimentin filaments judging from the replica and thin-sectioned image.

AB - We have visualized the nuclear pore complex (NPCx) three-dimensionally by quick-freeze, deep etching and suggest that NPCx is composed of four rings and associated filaments. The rings were located at the cytoplasmic side, at the nucleoplasmic side, and at the pore waist as well. Some of the filaments emanated toward the cytoplasm, some sprawled over the cytoplasmic face of the nuclear membrane, and some intervened the rings. The emanating filaments were considered to be vimentin filaments judging from the replica and thin-sectioned image.

KW - Deep-etching

KW - Intermediate filament

KW - Nuclear membrane

KW - Nuclear pore complex

KW - Osteosarcoma

KW - Quick-freeze

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M3 - Article

C2 - 2723571

AN - SCOPUS:0024487821

SN - 2050-5698

VL - 38

SP - 34

EP - 40

JO - Microscopy (Oxford, England)

JF - Microscopy (Oxford, England)

IS - 1

ER -

Quick-freeze, deep-etch visualization of the nuclear pore complex

Abstract

Keywords

ASJC Scopus subject areas

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