Quick-freeze, deep-etch visualization of the nuclear pore complex

Youhei Fujitani, Shouzou Higaki, Hajime Sawada, Kazushige Hirosawa

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We have visualized the nuclear pore complex (NPCx) three-dimensionally by quick-freeze, deep etching and suggest that NPCx is composed of four rings and associated filaments. The rings were located at the cytoplasmic side, at the nucleoplasmic side, and at the pore waist as well. Some of the filaments emanated toward the cytoplasm, some sprawled over the cytoplasmic face of the nuclear membrane, and some intervened the rings. The emanating filaments were considered to be vimentin filaments judging from the replica and thin-sectioned image.

Original languageEnglish
Pages (from-to)34-40
Number of pages7
JournalJournal of Electron Microscopy
Volume38
Issue number1
Publication statusPublished - 1989 Dec 1
Externally publishedYes

Keywords

  • Deep-etching
  • Intermediate filament
  • Nuclear membrane
  • Nuclear pore complex
  • Osteosarcoma
  • Quick-freeze

ASJC Scopus subject areas

  • Instrumentation

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