Abstract
STAT transcription factors are tyrosine phosphorylated upon cytokine stimulation and enter the nucleus to activate target genes. We show that Rac1 and a GTPase-activating protein, MgcRacGAP, bind directly to p-STAT5A and are required to promote its nuclear translocation. Using permeabilized cells, we find that nuclear translocation of purified p-STAT5A is dependent on the addition of GTP-bound Rac1, MgcRacGAP, importin α, and importin β. p-STAT3 also enters the nucleus via this transport machinery, and mutant STATs lacking the MgcRacGAP binding site do not enter the nucleus even after phosphorylation. We conclude that GTP-bound Rac1 and MgcRacGAP function as a nuclear transport chaperone for activated STATs.
| Original language | English |
|---|---|
| Pages (from-to) | 937-946 |
| Number of pages | 10 |
| Journal | Journal of Cell Biology |
| Volume | 175 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2006 Dec 18 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cell Biology
Cite this
Rac1 and a GTPase-activating protein, MgcRacGAP, are required for nuclear translocation of STAT transcription factors. / Kawashima, Toshiyuki; Ying, Chun Bao; Nomura, Yasushi; Moon, Yuseok; Tonozuka, Yukio; Minoshima, Yukinori; Hatori, Tomonori; Tsuchiya, Akiho; Kiyono, Mari; Nosaka, Tetsuya; Nakajima, Hideaki; Williams, David A.; Kitamura, Toshio.
In: Journal of Cell Biology, Vol. 175, No. 6, 18.12.2006, p. 937-946.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rac1 and a GTPase-activating protein, MgcRacGAP, are required for nuclear translocation of STAT transcription factors
AU - Kawashima, Toshiyuki
AU - Ying, Chun Bao
AU - Nomura, Yasushi
AU - Moon, Yuseok
AU - Tonozuka, Yukio
AU - Minoshima, Yukinori
AU - Hatori, Tomonori
AU - Tsuchiya, Akiho
AU - Kiyono, Mari
AU - Nosaka, Tetsuya
AU - Nakajima, Hideaki
AU - Williams, David A.
AU - Kitamura, Toshio
PY - 2006/12/18
Y1 - 2006/12/18
N2 - STAT transcription factors are tyrosine phosphorylated upon cytokine stimulation and enter the nucleus to activate target genes. We show that Rac1 and a GTPase-activating protein, MgcRacGAP, bind directly to p-STAT5A and are required to promote its nuclear translocation. Using permeabilized cells, we find that nuclear translocation of purified p-STAT5A is dependent on the addition of GTP-bound Rac1, MgcRacGAP, importin α, and importin β. p-STAT3 also enters the nucleus via this transport machinery, and mutant STATs lacking the MgcRacGAP binding site do not enter the nucleus even after phosphorylation. We conclude that GTP-bound Rac1 and MgcRacGAP function as a nuclear transport chaperone for activated STATs.
AB - STAT transcription factors are tyrosine phosphorylated upon cytokine stimulation and enter the nucleus to activate target genes. We show that Rac1 and a GTPase-activating protein, MgcRacGAP, bind directly to p-STAT5A and are required to promote its nuclear translocation. Using permeabilized cells, we find that nuclear translocation of purified p-STAT5A is dependent on the addition of GTP-bound Rac1, MgcRacGAP, importin α, and importin β. p-STAT3 also enters the nucleus via this transport machinery, and mutant STATs lacking the MgcRacGAP binding site do not enter the nucleus even after phosphorylation. We conclude that GTP-bound Rac1 and MgcRacGAP function as a nuclear transport chaperone for activated STATs.
UR - http://www.scopus.com/inward/record.url?scp=33845686162&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33845686162&partnerID=8YFLogxK
U2 - 10.1083/jcb.200604073
DO - 10.1083/jcb.200604073
M3 - Article
C2 - 17178910
AN - SCOPUS:33845686162
VL - 175
SP - 937
EP - 946
JO - Journal of Cell Biology
JF - Journal of Cell Biology
SN - 0021-9525
IS - 6
ER -