RANTES production in a conjunctival epithelial cell line

Kazumi Fukagawa, Hirohisa Saito, Kazuo Tsubota, Shigeto Shimmura, Hiroshi Tachimoto, Akira Akasawa, Yoshihisa Oguchi

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Purpose. Although corneal tissue damage in allergic ocular diseases is thought to be induced by inflammatory cells that infiltrate from conjunctival tissue, the mechanisms of recruiting these cells remain unclear. The objective of this study was to demonstrate whether conjunctival epithelial cells have the ability to produce 'regulated on activation, normal T-cell expressed and secreted' (RANTES). To test this hypothesis, we investigated RANTES expression in the conjunctival tissue and also RANTES production by cytokine stimulation in a human conjunctival epithelial cell line. Methods. We investigated the expression of the chemokine RANTES in conjunctival epithelium from two patients with atopic keratoconjunctivitis (AKC) and one patient with vernal keratoconjunctivitis (VKC) by using immunohistochemistry. We also investigated the production and suppression of RANTES from a human conjunctival epithelial cell line, Wong-Kilbourne-derived human conjunctiva (WK-hC) by using enzyme-linked immunosorbent assay (ELISA). Results. Conjunctival epithelium from a patient with AKC stained positively for RANTES. We found that tumor necrosis factor-α (TNF-α) induced de nero production of RANTES, and interferon-γ (IFN-γ) synergistically increased the TNF-α-dependent production of RANTES from WK-hC cells. Dexamethasone suppressed the RANTES production from the cell line. Conclusion. Taken together, human conjunctival epithelial cells were capable of producing RANTES in response to inflammatory stimuli such as TNF-α and may play a role in recruiting inflammatory cells such as eosinophils and T lymphocytes toward the ocular surface.

Original languageEnglish
Pages (from-to)564-570
Number of pages7
JournalCornea
Volume16
Issue number5
Publication statusPublished - 1997 Sep

Fingerprint

Epithelial Cells
T-Lymphocytes
Cell Line
Keratoconjunctivitis
Tumor Necrosis Factor-alpha
Conjunctiva
Epithelium
Allergic Conjunctivitis
Eye Diseases
Chemokines
Eosinophils
Interferons
Dexamethasone
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Cytokines

Keywords

  • Chemokine
  • Conjunctival epithelial cell
  • Interferon-γ
  • RANTES
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Fukagawa, K., Saito, H., Tsubota, K., Shimmura, S., Tachimoto, H., Akasawa, A., & Oguchi, Y. (1997). RANTES production in a conjunctival epithelial cell line. Cornea, 16(5), 564-570.

RANTES production in a conjunctival epithelial cell line. / Fukagawa, Kazumi; Saito, Hirohisa; Tsubota, Kazuo; Shimmura, Shigeto; Tachimoto, Hiroshi; Akasawa, Akira; Oguchi, Yoshihisa.

In: Cornea, Vol. 16, No. 5, 09.1997, p. 564-570.

Research output: Contribution to journalArticle

Fukagawa, K, Saito, H, Tsubota, K, Shimmura, S, Tachimoto, H, Akasawa, A & Oguchi, Y 1997, 'RANTES production in a conjunctival epithelial cell line', Cornea, vol. 16, no. 5, pp. 564-570.
Fukagawa K, Saito H, Tsubota K, Shimmura S, Tachimoto H, Akasawa A et al. RANTES production in a conjunctival epithelial cell line. Cornea. 1997 Sep;16(5):564-570.
Fukagawa, Kazumi ; Saito, Hirohisa ; Tsubota, Kazuo ; Shimmura, Shigeto ; Tachimoto, Hiroshi ; Akasawa, Akira ; Oguchi, Yoshihisa. / RANTES production in a conjunctival epithelial cell line. In: Cornea. 1997 ; Vol. 16, No. 5. pp. 564-570.
@article{65cff3e68b6e4b398ce55182ca2afb1c,
title = "RANTES production in a conjunctival epithelial cell line",
abstract = "Purpose. Although corneal tissue damage in allergic ocular diseases is thought to be induced by inflammatory cells that infiltrate from conjunctival tissue, the mechanisms of recruiting these cells remain unclear. The objective of this study was to demonstrate whether conjunctival epithelial cells have the ability to produce 'regulated on activation, normal T-cell expressed and secreted' (RANTES). To test this hypothesis, we investigated RANTES expression in the conjunctival tissue and also RANTES production by cytokine stimulation in a human conjunctival epithelial cell line. Methods. We investigated the expression of the chemokine RANTES in conjunctival epithelium from two patients with atopic keratoconjunctivitis (AKC) and one patient with vernal keratoconjunctivitis (VKC) by using immunohistochemistry. We also investigated the production and suppression of RANTES from a human conjunctival epithelial cell line, Wong-Kilbourne-derived human conjunctiva (WK-hC) by using enzyme-linked immunosorbent assay (ELISA). Results. Conjunctival epithelium from a patient with AKC stained positively for RANTES. We found that tumor necrosis factor-α (TNF-α) induced de nero production of RANTES, and interferon-γ (IFN-γ) synergistically increased the TNF-α-dependent production of RANTES from WK-hC cells. Dexamethasone suppressed the RANTES production from the cell line. Conclusion. Taken together, human conjunctival epithelial cells were capable of producing RANTES in response to inflammatory stimuli such as TNF-α and may play a role in recruiting inflammatory cells such as eosinophils and T lymphocytes toward the ocular surface.",
keywords = "Chemokine, Conjunctival epithelial cell, Interferon-γ, RANTES, Tumor necrosis factor-α",
author = "Kazumi Fukagawa and Hirohisa Saito and Kazuo Tsubota and Shigeto Shimmura and Hiroshi Tachimoto and Akira Akasawa and Yoshihisa Oguchi",
year = "1997",
month = "9",
language = "English",
volume = "16",
pages = "564--570",
journal = "Cornea",
issn = "0277-3740",
publisher = "Lippincott Williams and Wilkins",
number = "5",

}

TY - JOUR

T1 - RANTES production in a conjunctival epithelial cell line

AU - Fukagawa, Kazumi

AU - Saito, Hirohisa

AU - Tsubota, Kazuo

AU - Shimmura, Shigeto

AU - Tachimoto, Hiroshi

AU - Akasawa, Akira

AU - Oguchi, Yoshihisa

PY - 1997/9

Y1 - 1997/9

N2 - Purpose. Although corneal tissue damage in allergic ocular diseases is thought to be induced by inflammatory cells that infiltrate from conjunctival tissue, the mechanisms of recruiting these cells remain unclear. The objective of this study was to demonstrate whether conjunctival epithelial cells have the ability to produce 'regulated on activation, normal T-cell expressed and secreted' (RANTES). To test this hypothesis, we investigated RANTES expression in the conjunctival tissue and also RANTES production by cytokine stimulation in a human conjunctival epithelial cell line. Methods. We investigated the expression of the chemokine RANTES in conjunctival epithelium from two patients with atopic keratoconjunctivitis (AKC) and one patient with vernal keratoconjunctivitis (VKC) by using immunohistochemistry. We also investigated the production and suppression of RANTES from a human conjunctival epithelial cell line, Wong-Kilbourne-derived human conjunctiva (WK-hC) by using enzyme-linked immunosorbent assay (ELISA). Results. Conjunctival epithelium from a patient with AKC stained positively for RANTES. We found that tumor necrosis factor-α (TNF-α) induced de nero production of RANTES, and interferon-γ (IFN-γ) synergistically increased the TNF-α-dependent production of RANTES from WK-hC cells. Dexamethasone suppressed the RANTES production from the cell line. Conclusion. Taken together, human conjunctival epithelial cells were capable of producing RANTES in response to inflammatory stimuli such as TNF-α and may play a role in recruiting inflammatory cells such as eosinophils and T lymphocytes toward the ocular surface.

AB - Purpose. Although corneal tissue damage in allergic ocular diseases is thought to be induced by inflammatory cells that infiltrate from conjunctival tissue, the mechanisms of recruiting these cells remain unclear. The objective of this study was to demonstrate whether conjunctival epithelial cells have the ability to produce 'regulated on activation, normal T-cell expressed and secreted' (RANTES). To test this hypothesis, we investigated RANTES expression in the conjunctival tissue and also RANTES production by cytokine stimulation in a human conjunctival epithelial cell line. Methods. We investigated the expression of the chemokine RANTES in conjunctival epithelium from two patients with atopic keratoconjunctivitis (AKC) and one patient with vernal keratoconjunctivitis (VKC) by using immunohistochemistry. We also investigated the production and suppression of RANTES from a human conjunctival epithelial cell line, Wong-Kilbourne-derived human conjunctiva (WK-hC) by using enzyme-linked immunosorbent assay (ELISA). Results. Conjunctival epithelium from a patient with AKC stained positively for RANTES. We found that tumor necrosis factor-α (TNF-α) induced de nero production of RANTES, and interferon-γ (IFN-γ) synergistically increased the TNF-α-dependent production of RANTES from WK-hC cells. Dexamethasone suppressed the RANTES production from the cell line. Conclusion. Taken together, human conjunctival epithelial cells were capable of producing RANTES in response to inflammatory stimuli such as TNF-α and may play a role in recruiting inflammatory cells such as eosinophils and T lymphocytes toward the ocular surface.

KW - Chemokine

KW - Conjunctival epithelial cell

KW - Interferon-γ

KW - RANTES

KW - Tumor necrosis factor-α

UR - http://www.scopus.com/inward/record.url?scp=0030796405&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030796405&partnerID=8YFLogxK

M3 - Article

C2 - 9294691

AN - SCOPUS:0030796405

VL - 16

SP - 564

EP - 570

JO - Cornea

JF - Cornea

SN - 0277-3740

IS - 5

ER -