Rapid and accurate IC50 determination using logarithmic concentration generator

Yuta Abe, Hirotaka Sasaki, Toshihisa Osaki, Koki Kamiya, Ryuji Kawano, Norihisa Miki, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper proposes an effective methodology for the determination of the half maximal (50%) inhibitory concentration (IC50) by using microfluidic device that generates logarithmic concentration gradients. Dose-response curve is obtained with respect to the concentrations in a logarithmic scale. Although we have presented a high-throughput analysis of ATP binding cassette transporter (ABC-transporter) by using linear concentration generator [1] and fluorescent substrates [2], logarithmic concentration generator is demanded for rapid and accurate IC50 determination as shown in Fig. 1. We designed a microfluidic device composed of branching micro-channels where samples are divided and mixed to generate designated concentrations. Finally inhibition analysis of substrate transport was conducted by linear and logarithmic concentration generators. We found that logarithmic concentration generators are useful for the accurate determination of IC50 by a fewer number of tests than linear concentration generators.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages956-958
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012 Jan 1
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 2012 Oct 282012 Nov 1

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12/10/2812/11/1

Keywords

  • Drug screening
  • Microfluidics

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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