TY - JOUR
T1 - Rapid and label-free sensing of intermolecular interactions using compact optical diffusion sensor
AU - Kamata, Makoto
AU - Takaba, Yoshiaki
AU - Taguchi, Yoshihiro
AU - Nagasaka, Yuji
N1 - Funding Information:
The fabrication was performed in a clean room at the Global Nano Micro Technology Business Incubation Center (NANOBIC), Kawasaki, Japan with support from the Academic Consortium for Nano and Micro Fabrication of Four Universities (Keio University, Waseda University, Tokyo Institute of Technology, and The University of Tokyo). The fabrication of the sensing device was partially supported by Kyodo International, Inc. Authors would like to thank R. Shishido for her help in data analysis.
Funding Information:
This work was financially supported by JSPS KAKENHI grant numbers JP17J04445 , JP18H01388 .
Publisher Copyright:
© 2018 The Authors
PY - 2019/4
Y1 - 2019/4
N2 - Diffusion sensors for nano-sized materials dispersed in a solution are promising tools for investigating intermolecular interactions, which is an important task in a wide range of industrial and biomedical fields, such as the development of therapeutic products and diagnostic tools. For the development of the sensor applicable to point-of-care testing, an optofluidic sensor was proposed. The proposed sensor realizes a simple measurement in a short time using microscale manipulation by interferometrically-induced dielectrophoresis and optical detection. In this paper, the applicability of the proposed sensor to distinguish nanoscale differences in size was confirmed by measurements on a solution containing size-certified plain nano-beads (51 nm, 100 nm, 203 nm, 216 nm and 240 nm) with evaluating the uncertainties of the proposed sensor. Furthermore, measurements using 200-nm beads coated with functional groups (NH2 and biotin) and proteins (streptavidin and bovine serum albumin) demonstrated the applicability to the investigation of intermolecular interactions.
AB - Diffusion sensors for nano-sized materials dispersed in a solution are promising tools for investigating intermolecular interactions, which is an important task in a wide range of industrial and biomedical fields, such as the development of therapeutic products and diagnostic tools. For the development of the sensor applicable to point-of-care testing, an optofluidic sensor was proposed. The proposed sensor realizes a simple measurement in a short time using microscale manipulation by interferometrically-induced dielectrophoresis and optical detection. In this paper, the applicability of the proposed sensor to distinguish nanoscale differences in size was confirmed by measurements on a solution containing size-certified plain nano-beads (51 nm, 100 nm, 203 nm, 216 nm and 240 nm) with evaluating the uncertainties of the proposed sensor. Furthermore, measurements using 200-nm beads coated with functional groups (NH2 and biotin) and proteins (streptavidin and bovine serum albumin) demonstrated the applicability to the investigation of intermolecular interactions.
KW - Diffusion coefficient
KW - Intermolecular interaction
KW - Label-free sensing
KW - Laser-induced dielectrophoresis
KW - Optofluidic sensor
KW - Rapid measurement
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U2 - 10.1016/j.ijheatmasstransfer.2018.12.088
DO - 10.1016/j.ijheatmasstransfer.2018.12.088
M3 - Article
AN - SCOPUS:85058643698
VL - 133
SP - 73
EP - 79
JO - International Journal of Heat and Mass Transfer
JF - International Journal of Heat and Mass Transfer
SN - 0017-9310
ER -