Rapid and Resilient Detection of Toxin Pore Formation Using a Lipid Bilayer Array

Yoshihisa Ito, Toshihisa Osaki, Koki Kamiya, Tetsuya Yamada, Norihisa Miki, Shoji Takeuchi

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

An artificial cell membrane is applied to study the pore formation mechanisms of bacterial pore-forming toxins for therapeutic applications. Electrical monitoring of ionic current across the membrane provides information on the pore formation process of toxins at the single pore level, as well as the pore characteristics such as dimensions and ionic selectivity. However, the efficiency of pore formation detection largely depends on the encounter probability of toxin to the membrane and the fragility of the membrane. This study presents a bilayer lipid membrane array that parallelizes 4 or 16 sets of sensing elements composed of pairs of a membrane and a series electrical resistor. The series resistor prevents current overflow attributed to membrane rupture, and enables current monitoring of the parallelized membranes with a single detector. The array system shortens detection time of a pore-forming protein and improves temporal stability. The current signature represents the states of pore formation and rupture at respective membranes. The developed system will help in understanding the toxic activity of pore-forming toxins.

Original languageEnglish
Article number2005550
JournalSmall
Volume16
Issue number49
DOIs
Publication statusPublished - 2020 Dec 10

Keywords

  • electrophysiological analysis
  • planar bilayer lipid membranes
  • pore-forming proteins
  • stochastic process

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)

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