Rapid antibody selection by mRNA display on a microfluidic chip

Noriko Tabata, Yuko Sakuma, Yumiko Honda, Nobuhide Doi, Hideaki Takashima, Etsuko Miyamoto-Sato, Hiroshi Yanagawa

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

In vitro antibody-display technologies are powerful approaches for isolating monoclonal antibodies from recombinant antibody libraries. However, these display techniques require several rounds of affinity selection which is time-consuming. Here, we combined mRNA display with a microfluidic system for in vitro selection and evolution of antibodies and achieved ultrahigh enrichment efficiency of 106-to 108-fold per round. After only one or two rounds of selection, antibodies with high affinity and specificity were obtained from naïve and randomized single-chain Fv libraries of ∼1012 molecules. Furthermore, we confirmed that not only protein-protein (antigen-antibody) interactions, but also protein-DNA and protein-drug interactions were selected with ultrahigh efficiencies. This method will facilitate high-throughput preparation of antibodies and identification of protein interactions in proteomic and therapeutic fields.

Original languageEnglish
Article numbere64
JournalNucleic acids research
Volume37
Issue number8
DOIs
Publication statusPublished - 2009 May 29

    Fingerprint

ASJC Scopus subject areas

  • Genetics

Cite this

Tabata, N., Sakuma, Y., Honda, Y., Doi, N., Takashima, H., Miyamoto-Sato, E., & Yanagawa, H. (2009). Rapid antibody selection by mRNA display on a microfluidic chip. Nucleic acids research, 37(8), [e64]. https://doi.org/10.1093/nar/gkp184