Rapid antibody selection by mRNA display on a microfluidic chip

Noriko Tabata, Yuko Sakuma, Yumiko Honda, Nobuhide Doi, Hideaki Takashima, Etsuko Miyamoto-Sato, Hiroshi Yanagawa

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

In vitro antibody-display technologies are powerful approaches for isolating monoclonal antibodies from recombinant antibody libraries. However, these display techniques require several rounds of affinity selection which is time-consuming. Here, we combined mRNA display with a microfluidic system for in vitro selection and evolution of antibodies and achieved ultrahigh enrichment efficiency of 106-to 108-fold per round. After only one or two rounds of selection, antibodies with high affinity and specificity were obtained from naïve and randomized single-chain Fv libraries of ∼1012 molecules. Furthermore, we confirmed that not only protein-protein (antigen-antibody) interactions, but also protein-DNA and protein-drug interactions were selected with ultrahigh efficiencies. This method will facilitate high-throughput preparation of antibodies and identification of protein interactions in proteomic and therapeutic fields.

Original languageEnglish
Article numbere64
JournalNucleic Acids Research
Volume37
Issue number8
DOIs
Publication statusPublished - 2009

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Microfluidics
Messenger RNA
Antibodies
Proteins
Single-Chain Antibodies
Drug Interactions
Proteomics
Monoclonal Antibodies
Technology
Antigens
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Tabata, N., Sakuma, Y., Honda, Y., Doi, N., Takashima, H., Miyamoto-Sato, E., & Yanagawa, H. (2009). Rapid antibody selection by mRNA display on a microfluidic chip. Nucleic Acids Research, 37(8), [e64]. https://doi.org/10.1093/nar/gkp184

Rapid antibody selection by mRNA display on a microfluidic chip. / Tabata, Noriko; Sakuma, Yuko; Honda, Yumiko; Doi, Nobuhide; Takashima, Hideaki; Miyamoto-Sato, Etsuko; Yanagawa, Hiroshi.

In: Nucleic Acids Research, Vol. 37, No. 8, e64, 2009.

Research output: Contribution to journalArticle

Tabata, N, Sakuma, Y, Honda, Y, Doi, N, Takashima, H, Miyamoto-Sato, E & Yanagawa, H 2009, 'Rapid antibody selection by mRNA display on a microfluidic chip', Nucleic Acids Research, vol. 37, no. 8, e64. https://doi.org/10.1093/nar/gkp184
Tabata N, Sakuma Y, Honda Y, Doi N, Takashima H, Miyamoto-Sato E et al. Rapid antibody selection by mRNA display on a microfluidic chip. Nucleic Acids Research. 2009;37(8). e64. https://doi.org/10.1093/nar/gkp184
Tabata, Noriko ; Sakuma, Yuko ; Honda, Yumiko ; Doi, Nobuhide ; Takashima, Hideaki ; Miyamoto-Sato, Etsuko ; Yanagawa, Hiroshi. / Rapid antibody selection by mRNA display on a microfluidic chip. In: Nucleic Acids Research. 2009 ; Vol. 37, No. 8.
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