Rapid detection of mecA gene by nested PCR for diagnosis of methicillin resistance in Staphylococcus aureus

Yuukou Kitagawa, M. Ueda, N. Ando, K. Ishibiki, M. Kitajima, Y. Kabayashi, Tsunenori Arai

Research output: Contribution to journalArticle

Abstract

In recent years, the most common causative organism of hospital infections has been methicillin resistant Staphylococcus aureus (MRSA). The major mechanism of beta-lactam resistance in MRSA is attributed to the production of a specific penicillin binding protein (PBP2'), which is a product of mecA gene, with extremely low binding affinities to beta-lactams. In the present study, we have established a rapid identification method of MRSA by sensitive detection of mecA gene using nested PCR. Nested PCR method amplifying the target DNA in two steps enhanced the efficiency of the second round amplification. By means of this method, mecA gene was successfully detected in clinical samples, such as blood, pus, sputum and feces within 3-4 hrs. Rapid diagnosis of MRSA-bacteremia is particularly important for prevention of sever systemic infection. There are some strains of S. aureus which possess mecA gene in spite of low minimal inhibitory concentration of DMPPC. In these strains expression of mecA gene is induced by contact of beta-lactams and they obtain methicillin resistance. Using nested PCR method, these latent MRSA are rapidly and certainly detectable. This method should be useful for early and effective detection of MRSA hospital infections.

Original languageEnglish
Pages (from-to)914-917
Number of pages4
JournalNippon Geka Gakkai zasshi
Volume93
Issue number9
Publication statusPublished - 1992 Sep

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Methicillin Resistance
Methicillin-Resistant Staphylococcus aureus
Staphylococcus aureus
Polymerase Chain Reaction
Genes
beta-Lactams
Cross Infection
beta-Lactam Resistance
Penicillin-Binding Proteins
Suppuration
Bacteremia
Sputum
Feces
Gene Expression
DNA
Infection

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Kitagawa, Y., Ueda, M., Ando, N., Ishibiki, K., Kitajima, M., Kabayashi, Y., & Arai, T. (1992). Rapid detection of mecA gene by nested PCR for diagnosis of methicillin resistance in Staphylococcus aureus. Nippon Geka Gakkai zasshi, 93(9), 914-917.

Rapid detection of mecA gene by nested PCR for diagnosis of methicillin resistance in Staphylococcus aureus. / Kitagawa, Yuukou; Ueda, M.; Ando, N.; Ishibiki, K.; Kitajima, M.; Kabayashi, Y.; Arai, Tsunenori.

In: Nippon Geka Gakkai zasshi, Vol. 93, No. 9, 09.1992, p. 914-917.

Research output: Contribution to journalArticle

Kitagawa, Y, Ueda, M, Ando, N, Ishibiki, K, Kitajima, M, Kabayashi, Y & Arai, T 1992, 'Rapid detection of mecA gene by nested PCR for diagnosis of methicillin resistance in Staphylococcus aureus', Nippon Geka Gakkai zasshi, vol. 93, no. 9, pp. 914-917.
Kitagawa, Yuukou ; Ueda, M. ; Ando, N. ; Ishibiki, K. ; Kitajima, M. ; Kabayashi, Y. ; Arai, Tsunenori. / Rapid detection of mecA gene by nested PCR for diagnosis of methicillin resistance in Staphylococcus aureus. In: Nippon Geka Gakkai zasshi. 1992 ; Vol. 93, No. 9. pp. 914-917.
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