Rapid diagnosis of methicillin-resistant Staphylococcus aureus bacteremia by nested polymerase chain reaction

Yuukou Kitagawa, M. Ueda, N. Ando, M. Endo, K. Ishibiki, Y. Kobayashi, Tsunenori Arai, M. Kitajima

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Objective: The purpose of this study was to establish a rapid and sensitive diagnostic method for methicillin-resistant Staphylococcus aureus (MRSA) bacteremia in postoperative patients. Summary Background Data: As a result of diffusion and abuse of third-generation cephalosporin antibiotics in the 1980s in Japan, an outbreak of MRSA infection has been posed. In the field of surgery, severe postoperative infections with MRSA such as MRSA bacteremia, which may lead to multiple organ failure, have emerged with a high mortality. Methods: Thirty-five patients with high fever (above 38.5°C) or watery diarrhea or both within 2 weeks after gastrointestinal major surgery and 6 healthy volunteers were examined. Nested polymerase chain reaction was used to detect meca and toxic shock syndrome toxin-1 (TSST-1) genes in blood specimens. Results: The meca and TSST-1 genes were not detected in the blood samples of any of the six healthy volunteers. In all 12 samples from which MRSA colonies were isolated by blood culture, mecA and TSST-1 genes were detected. Although it took at least 48 hours to identify MRSA by the blood culture method, the presence of mecA and TSST-1 genes was determined by nested polymerase chain reaction method within only 3 to 4 hours after blood sampling. Conclusions: This method, as a sensitive and rapid monitoring system for MRSA bacteremia, would be clinically beneficial for prevention of cross infection and for early determination of appropriate treatment for infected patients.

Original languageEnglish
Pages (from-to)665-671
Number of pages7
JournalAnnals of Surgery
Volume224
Issue number5
DOIs
Publication statusPublished - 1996

Fingerprint

Methicillin-Resistant Staphylococcus aureus
Bacteremia
Polymerase Chain Reaction
Genes
Healthy Volunteers
Multiple Organ Failure
Cephalosporins
Cross Infection
Infection
Disease Outbreaks
Diarrhea
Japan
Fever
Mortality
Staphylococcal enterotoxin F

ASJC Scopus subject areas

  • Surgery

Cite this

Rapid diagnosis of methicillin-resistant Staphylococcus aureus bacteremia by nested polymerase chain reaction. / Kitagawa, Yuukou; Ueda, M.; Ando, N.; Endo, M.; Ishibiki, K.; Kobayashi, Y.; Arai, Tsunenori; Kitajima, M.

In: Annals of Surgery, Vol. 224, No. 5, 1996, p. 665-671.

Research output: Contribution to journalArticle

Kitagawa, Yuukou ; Ueda, M. ; Ando, N. ; Endo, M. ; Ishibiki, K. ; Kobayashi, Y. ; Arai, Tsunenori ; Kitajima, M. / Rapid diagnosis of methicillin-resistant Staphylococcus aureus bacteremia by nested polymerase chain reaction. In: Annals of Surgery. 1996 ; Vol. 224, No. 5. pp. 665-671.
@article{79a3ca655a8e4ad98e0bfec62445bdcb,
title = "Rapid diagnosis of methicillin-resistant Staphylococcus aureus bacteremia by nested polymerase chain reaction",
abstract = "Objective: The purpose of this study was to establish a rapid and sensitive diagnostic method for methicillin-resistant Staphylococcus aureus (MRSA) bacteremia in postoperative patients. Summary Background Data: As a result of diffusion and abuse of third-generation cephalosporin antibiotics in the 1980s in Japan, an outbreak of MRSA infection has been posed. In the field of surgery, severe postoperative infections with MRSA such as MRSA bacteremia, which may lead to multiple organ failure, have emerged with a high mortality. Methods: Thirty-five patients with high fever (above 38.5°C) or watery diarrhea or both within 2 weeks after gastrointestinal major surgery and 6 healthy volunteers were examined. Nested polymerase chain reaction was used to detect meca and toxic shock syndrome toxin-1 (TSST-1) genes in blood specimens. Results: The meca and TSST-1 genes were not detected in the blood samples of any of the six healthy volunteers. In all 12 samples from which MRSA colonies were isolated by blood culture, mecA and TSST-1 genes were detected. Although it took at least 48 hours to identify MRSA by the blood culture method, the presence of mecA and TSST-1 genes was determined by nested polymerase chain reaction method within only 3 to 4 hours after blood sampling. Conclusions: This method, as a sensitive and rapid monitoring system for MRSA bacteremia, would be clinically beneficial for prevention of cross infection and for early determination of appropriate treatment for infected patients.",
author = "Yuukou Kitagawa and M. Ueda and N. Ando and M. Endo and K. Ishibiki and Y. Kobayashi and Tsunenori Arai and M. Kitajima",
year = "1996",
doi = "10.1097/00000658-199611000-00010",
language = "English",
volume = "224",
pages = "665--671",
journal = "Annals of Surgery",
issn = "0003-4932",
publisher = "Lippincott Williams and Wilkins",
number = "5",

}

TY - JOUR

T1 - Rapid diagnosis of methicillin-resistant Staphylococcus aureus bacteremia by nested polymerase chain reaction

AU - Kitagawa, Yuukou

AU - Ueda, M.

AU - Ando, N.

AU - Endo, M.

AU - Ishibiki, K.

AU - Kobayashi, Y.

AU - Arai, Tsunenori

AU - Kitajima, M.

PY - 1996

Y1 - 1996

N2 - Objective: The purpose of this study was to establish a rapid and sensitive diagnostic method for methicillin-resistant Staphylococcus aureus (MRSA) bacteremia in postoperative patients. Summary Background Data: As a result of diffusion and abuse of third-generation cephalosporin antibiotics in the 1980s in Japan, an outbreak of MRSA infection has been posed. In the field of surgery, severe postoperative infections with MRSA such as MRSA bacteremia, which may lead to multiple organ failure, have emerged with a high mortality. Methods: Thirty-five patients with high fever (above 38.5°C) or watery diarrhea or both within 2 weeks after gastrointestinal major surgery and 6 healthy volunteers were examined. Nested polymerase chain reaction was used to detect meca and toxic shock syndrome toxin-1 (TSST-1) genes in blood specimens. Results: The meca and TSST-1 genes were not detected in the blood samples of any of the six healthy volunteers. In all 12 samples from which MRSA colonies were isolated by blood culture, mecA and TSST-1 genes were detected. Although it took at least 48 hours to identify MRSA by the blood culture method, the presence of mecA and TSST-1 genes was determined by nested polymerase chain reaction method within only 3 to 4 hours after blood sampling. Conclusions: This method, as a sensitive and rapid monitoring system for MRSA bacteremia, would be clinically beneficial for prevention of cross infection and for early determination of appropriate treatment for infected patients.

AB - Objective: The purpose of this study was to establish a rapid and sensitive diagnostic method for methicillin-resistant Staphylococcus aureus (MRSA) bacteremia in postoperative patients. Summary Background Data: As a result of diffusion and abuse of third-generation cephalosporin antibiotics in the 1980s in Japan, an outbreak of MRSA infection has been posed. In the field of surgery, severe postoperative infections with MRSA such as MRSA bacteremia, which may lead to multiple organ failure, have emerged with a high mortality. Methods: Thirty-five patients with high fever (above 38.5°C) or watery diarrhea or both within 2 weeks after gastrointestinal major surgery and 6 healthy volunteers were examined. Nested polymerase chain reaction was used to detect meca and toxic shock syndrome toxin-1 (TSST-1) genes in blood specimens. Results: The meca and TSST-1 genes were not detected in the blood samples of any of the six healthy volunteers. In all 12 samples from which MRSA colonies were isolated by blood culture, mecA and TSST-1 genes were detected. Although it took at least 48 hours to identify MRSA by the blood culture method, the presence of mecA and TSST-1 genes was determined by nested polymerase chain reaction method within only 3 to 4 hours after blood sampling. Conclusions: This method, as a sensitive and rapid monitoring system for MRSA bacteremia, would be clinically beneficial for prevention of cross infection and for early determination of appropriate treatment for infected patients.

UR - http://www.scopus.com/inward/record.url?scp=0029804762&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029804762&partnerID=8YFLogxK

U2 - 10.1097/00000658-199611000-00010

DO - 10.1097/00000658-199611000-00010

M3 - Article

C2 - 8916881

AN - SCOPUS:0029804762

VL - 224

SP - 665

EP - 671

JO - Annals of Surgery

JF - Annals of Surgery

SN - 0003-4932

IS - 5

ER -