Rapid differentiation of human pluripotent stem cells into functional neurons by mRNAs encoding transcription factors

Sravan Kumar Goparaju, Kazuhisa Kohda, Keiji Ibata, Atsumi Soma, Yuki Nakatake, Tomohiko Akiyama, Shunichi Wakabayashi, Misako Matsushita, Miki Sakota, Hiromi Kimura, Michisuke Yuzaki, Shigeru Ko, Minoru Ko

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Efficient differentiation of human pluripotent stem cells (hPSCs) into neurons is paramount for disease modeling, drug screening, and cell transplantation therapy in regenerative medicine. In this manuscript, we report the capability of five transcription factors (TFs) toward this aim: NEUROG1, NEUROG2, NEUROG3, NEUROD1, and NEUROD2. In contrast to previous methods that have shortcomings in their speed and efficiency, a cocktail of these TFs as synthetic mRNAs can differentiate hPSCs into neurons in 7 days, judged by calcium imaging and electrophysiology. They exhibit motor neuron phenotypes based on immunostaining. These results indicate the establishment of a novel method for rapid, efficient, and footprint-free differentiation of functional neurons from hPSCs.

Original languageEnglish
Article number42367
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - 2017 Feb 13

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Pluripotent Stem Cells
Transcription Factors
Neurons
Messenger RNA
Preclinical Drug Evaluations
Regenerative Medicine
Electrophysiology
Cell Transplantation
Motor Neurons
Cell- and Tissue-Based Therapy
Calcium
Phenotype

ASJC Scopus subject areas

  • General

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Rapid differentiation of human pluripotent stem cells into functional neurons by mRNAs encoding transcription factors. / Goparaju, Sravan Kumar; Kohda, Kazuhisa; Ibata, Keiji; Soma, Atsumi; Nakatake, Yuki; Akiyama, Tomohiko; Wakabayashi, Shunichi; Matsushita, Misako; Sakota, Miki; Kimura, Hiromi; Yuzaki, Michisuke; Ko, Shigeru; Ko, Minoru.

In: Scientific Reports, Vol. 7, 42367, 13.02.2017.

Research output: Contribution to journalArticle

Goparaju, Sravan Kumar ; Kohda, Kazuhisa ; Ibata, Keiji ; Soma, Atsumi ; Nakatake, Yuki ; Akiyama, Tomohiko ; Wakabayashi, Shunichi ; Matsushita, Misako ; Sakota, Miki ; Kimura, Hiromi ; Yuzaki, Michisuke ; Ko, Shigeru ; Ko, Minoru. / Rapid differentiation of human pluripotent stem cells into functional neurons by mRNAs encoding transcription factors. In: Scientific Reports. 2017 ; Vol. 7.
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AU - Akiyama, Tomohiko

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