Rapid formation of size-controlled 3-dimensional hetero-spheroid using micro-rotational flow

T. Kodama, H. Ota, N. Miki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper, we demonstrate formation of size-controlled 3-dimensional hetero-spheroids using a micro-fluidic device in approximately 2 min. The device collects cells in the center of a micro-chamber using micro-rotational flow as shown in Fig. 1. In previous work, we used this device to generate size-controlled HepG2 spheroids [1]. We applied it to form hetero-spheroids consisting of HepG2 and HUVEC. We experimentally found that the spheroid sizes could be controlled by the cell densities of the media independent of the ratio of HepG2 and HUVEC. Uniform dispersion of HepG2 and HUVEC in the formed spheroid was also experimentally verified.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages359-361
Number of pages3
Publication statusPublished - 2011 Dec 1
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2011 Oct 22011 Oct 6

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume1

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period11/10/211/10/6

Keywords

  • Bio MEMS
  • Hetero-spheroid
  • Micro-fluidics
  • Micro-rotational flow

ASJC Scopus subject areas

  • Control and Systems Engineering

Fingerprint Dive into the research topics of 'Rapid formation of size-controlled 3-dimensional hetero-spheroid using micro-rotational flow'. Together they form a unique fingerprint.

  • Cite this

    Kodama, T., Ota, H., & Miki, N. (2011). Rapid formation of size-controlled 3-dimensional hetero-spheroid using micro-rotational flow. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 359-361). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 1).