Rapid perfusion system for inhibition investigation of membrane proteins in planar lipid bilayer

Y. Tsuji, R. Kawano, T. Osaki, K. Kamiya, Norihisa Miki, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper describes measurement system of membrane proteins with a rapid perfusion capability using microfluidic channels. In the previous report, we successfully recorded channel conductance of alpha-hemolysine (αHL) in bilayer lipid membranes (BLMs) using droplets contacting method with PDWC (parylene double well chip). This method is one of the easiest ways for preparing the BLMs, just pipetting droplets of the solution. The drawback of the droplet-based systems is the incapability of exchanging solution. To efficiently test the effect of inhibitors and promoters of membrane proteins in drug discovery, the solution exchange is mandatory. In this study, we proposed the concept of solution exchange in the system using microfluidic channel. We demonstrated rapid perfusion that is capable of exchanging the solution within 20 s in this droplets contacting system. We experimentally evaluated the system in terms of the flow rate and the BLM stability and demonstrated the binding assay of a membrane protein using its blockers. We believe that this method is necessary for efficient drug screening in our droplet-based system.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages683-685
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 2012 Oct 282012 Nov 1

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12/10/2812/11/1

Fingerprint

Lipid bilayers
Membrane Proteins
Proteins
Membranes
Membrane Lipids
Microfluidics
Ion exchange
Assays
Screening
Flow rate
Pharmaceutical Preparations

Keywords

  • Bilayer lipid membranes
  • Droplets contacting method
  • Parylene double well chip
  • Solution exchange

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Tsuji, Y., Kawano, R., Osaki, T., Kamiya, K., Miki, N., & Takeuchi, S. (2012). Rapid perfusion system for inhibition investigation of membrane proteins in planar lipid bilayer. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 683-685). Chemical and Biological Microsystems Society.

Rapid perfusion system for inhibition investigation of membrane proteins in planar lipid bilayer. / Tsuji, Y.; Kawano, R.; Osaki, T.; Kamiya, K.; Miki, Norihisa; Takeuchi, S.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 683-685.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tsuji, Y, Kawano, R, Osaki, T, Kamiya, K, Miki, N & Takeuchi, S 2012, Rapid perfusion system for inhibition investigation of membrane proteins in planar lipid bilayer. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, pp. 683-685, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 12/10/28.
Tsuji Y, Kawano R, Osaki T, Kamiya K, Miki N, Takeuchi S. Rapid perfusion system for inhibition investigation of membrane proteins in planar lipid bilayer. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 683-685
Tsuji, Y. ; Kawano, R. ; Osaki, T. ; Kamiya, K. ; Miki, Norihisa ; Takeuchi, S. / Rapid perfusion system for inhibition investigation of membrane proteins in planar lipid bilayer. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 683-685
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