Rapid replacement of somatic linker histones with the oocyte-specific linker histone H1foo in nuclear transfer

Takahide Teranishi, Mamoru Tanaka, Shingo Kimoto, Yukiko Ono, Kei Miyakoshi, Tomohiro Kono, Yasunori Yoshimura

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

The most distinctive feature of oocyte-specific linker histones is the specific timing of their expression during embryonic development. In Xenopus nuclear transfer, somatic linker histones in the donor nucleus are replaced with oocyte-specific linker histone B4, leading to the involvement of oocyte-specific linker histones in nuclear reprogramming. We recently have discovered a mouse oocyte-specific linker histone, named H1foo, and demonstrated its expression pattern in normal preimplantation embryos. The present study was undertaken to determine whether the replacement of somatic linker histones with H1foo occurs during the process of mouse nuclear transfer. H1foo was detected in the donor nucleus soon after transplantation. Thereafter, H1foo was restricted to the chromatin in up to two-cell stage embryos. After fusion of an oocyte with a cell expressing GFP (green fluorescent protein)-tagged somatic linker histone H1c, immediate release of H1c in the donor nucleus was observed. In addition, we used fluorescence recovery after photobleaching (FRAP), and found that H1foo is more mobile than H1c in living cells. The greater mobility of H1foo may contribute to its rapid replacement and decreased stability of the embryonic chromatin structure. These results suggest that rapid replacement of H1c with H1foo may play an important role in nuclear remodeling.

Original languageEnglish
Pages (from-to)76-86
Number of pages11
JournalDevelopmental Biology
Volume266
Issue number1
DOIs
Publication statusPublished - 2004 Feb 1

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Histones
Oocytes
Chromatin
Embryonic Structures
Fluorescence Recovery After Photobleaching
Blastocyst
Xenopus
Green Fluorescent Proteins
Embryonic Development
Transplantation

Keywords

  • Fluorescence recovery after photobleaching (FRAP)
  • H1foo
  • Linker histone
  • Nuclear remodeling

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Rapid replacement of somatic linker histones with the oocyte-specific linker histone H1foo in nuclear transfer. / Teranishi, Takahide; Tanaka, Mamoru; Kimoto, Shingo; Ono, Yukiko; Miyakoshi, Kei; Kono, Tomohiro; Yoshimura, Yasunori.

In: Developmental Biology, Vol. 266, No. 1, 01.02.2004, p. 76-86.

Research output: Contribution to journalArticle

Teranishi, Takahide ; Tanaka, Mamoru ; Kimoto, Shingo ; Ono, Yukiko ; Miyakoshi, Kei ; Kono, Tomohiro ; Yoshimura, Yasunori. / Rapid replacement of somatic linker histones with the oocyte-specific linker histone H1foo in nuclear transfer. In: Developmental Biology. 2004 ; Vol. 266, No. 1. pp. 76-86.
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