Rat ClNC/gro

A novel mediator for locomotive and secretagogue activation of neutrophils in vivo

Hidekazu Suzuki, M. Suematsu, S. Miura, Y. Y. Liu, Kenji Watanabe, M. Miyasaka, S. Tsurufuji, M. Tsuchiya

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The effects of rat CINC/gro, a member of the interleukin-8 family, on the endothelium-neutrophil interaction and transendothelial macromolecular leakage were studied in rat mesenteric microcirculation. Thirty minutes after superfusion with 10 nM CINC/gro, the number of neutrophils adherent to the venular endothelium and those migrated across the venules were significantly increased with a concomitant elevation of luminol-dependent chemiluminescence at the site of adhesion. Transendothelial macromolecular leakage as assessed by the relative length of venular wall stained with monastral blue B was also increased at 30 min after the start of CINC/gro superfusion. Pretreatments with a CD18-directed monoclonal antibody, WT-3 (1 mg/kg), significantly attenuated the increase in number of adherent and migrated neutrophils, the increase in luminol-dependent chemiluminescence, and the venular macromolecular leakage after the application of CINC/gro. These data suggest that CINC/gro is a novel stimulator that evokes not only locomotive but also secretagogue activation of neutrophils via a CD18-dependent mechanism in vivo.

Original languageEnglish
Pages (from-to)652-657
Number of pages6
JournalJournal of Leukocyte Biology
Volume55
Issue number5
Publication statusPublished - 1994

Fingerprint

Neutrophil Activation
Luminol
Neutrophils
Luminescence
Endothelium
Venules
Microcirculation
Interleukin-8
Monoclonal Antibodies

Keywords

  • β-integrin
  • Free radical
  • Interleukin 8
  • Microcirculation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Suzuki, H., Suematsu, M., Miura, S., Liu, Y. Y., Watanabe, K., Miyasaka, M., ... Tsuchiya, M. (1994). Rat ClNC/gro: A novel mediator for locomotive and secretagogue activation of neutrophils in vivo. Journal of Leukocyte Biology, 55(5), 652-657.

Rat ClNC/gro : A novel mediator for locomotive and secretagogue activation of neutrophils in vivo. / Suzuki, Hidekazu; Suematsu, M.; Miura, S.; Liu, Y. Y.; Watanabe, Kenji; Miyasaka, M.; Tsurufuji, S.; Tsuchiya, M.

In: Journal of Leukocyte Biology, Vol. 55, No. 5, 1994, p. 652-657.

Research output: Contribution to journalArticle

Suzuki, H, Suematsu, M, Miura, S, Liu, YY, Watanabe, K, Miyasaka, M, Tsurufuji, S & Tsuchiya, M 1994, 'Rat ClNC/gro: A novel mediator for locomotive and secretagogue activation of neutrophils in vivo', Journal of Leukocyte Biology, vol. 55, no. 5, pp. 652-657.
Suzuki H, Suematsu M, Miura S, Liu YY, Watanabe K, Miyasaka M et al. Rat ClNC/gro: A novel mediator for locomotive and secretagogue activation of neutrophils in vivo. Journal of Leukocyte Biology. 1994;55(5):652-657.
Suzuki, Hidekazu ; Suematsu, M. ; Miura, S. ; Liu, Y. Y. ; Watanabe, Kenji ; Miyasaka, M. ; Tsurufuji, S. ; Tsuchiya, M. / Rat ClNC/gro : A novel mediator for locomotive and secretagogue activation of neutrophils in vivo. In: Journal of Leukocyte Biology. 1994 ; Vol. 55, No. 5. pp. 652-657.
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