Rat ClNC/gro: A novel mediator for locomotive and secretagogue activation of neutrophils in vivo

H. Suzuki, M. Suematsu, S. Miura, Y. Y. Liu, K. Watanabe, M. Miyasaka, S. Tsurufuji, M. Tsuchiya

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Abstract

The effects of rat CINC/gro, a member of the interleukin-8 family, on the endothelium-neutrophil interaction and transendothelial macromolecular leakage were studied in rat mesenteric microcirculation. Thirty minutes after superfusion with 10 nM CINC/gro, the number of neutrophils adherent to the venular endothelium and those migrated across the venules were significantly increased with a concomitant elevation of luminol-dependent chemiluminescence at the site of adhesion. Transendothelial macromolecular leakage as assessed by the relative length of venular wall stained with monastral blue B was also increased at 30 min after the start of CINC/gro superfusion. Pretreatments with a CD18-directed monoclonal antibody, WT-3 (1 mg/kg), significantly attenuated the increase in number of adherent and migrated neutrophils, the increase in luminol-dependent chemiluminescence, and the venular macromolecular leakage after the application of CINC/gro. These data suggest that CINC/gro is a novel stimulator that evokes not only locomotive but also secretagogue activation of neutrophils via a CD18-dependent mechanism in vivo.

Original languageEnglish
Pages (from-to)652-657
Number of pages6
JournalJournal of Leukocyte Biology
Volume55
Issue number5
DOIs
Publication statusPublished - 1994 Jan 1

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Keywords

  • Free radical
  • Interleukin 8
  • Microcirculation
  • β-integrin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

Cite this

Suzuki, H., Suematsu, M., Miura, S., Liu, Y. Y., Watanabe, K., Miyasaka, M., Tsurufuji, S., & Tsuchiya, M. (1994). Rat ClNC/gro: A novel mediator for locomotive and secretagogue activation of neutrophils in vivo. Journal of Leukocyte Biology, 55(5), 652-657. https://doi.org/10.1002/jlb.55.5.652