Rational design of boron dipyrromethene (BODIPY)-based photobleaching- resistant fluorophores applicable to a protein dynamics study

Toru Komatsu, Daihi Oushiki, Aoi Takeda, Masaki Miyamura, Tasuku Ueno, Takuya Terai, Kenjiro Hanaoka, Yasuteru Urano, Tomoko Mineno, Tetsuo Nagano

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

We studied the photobleaching of a library of boron dipyrromethene (BODIPY) derivatives with a range of electron densities, and found that the photobleaching rate is influenced by the electron-withdrawing capacity of the substituents. Electron-deficient BODIPYs generated less singlet oxygen, were less reactive to singlet oxygen, and were highly resistant to photobleaching. We confirmed the utility of one of these fluorophores, 2,6-diCO2R-BDP, for visualizing EGF receptor dynamics in cells expressing an SNAP-tagged EGF receptor.

Original languageEnglish
Pages (from-to)10055-10057
Number of pages3
JournalChemical Communications
Volume47
Issue number36
DOIs
Publication statusPublished - 2011 Sep 28
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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