Re-expression of functional P-selectin molecules on the endothelial cell surface by repeated stimulation with thrombin

Hideto Kameda, Ikuo Morita, Makoto Handa, Junichi Kaburaki, Tadashi Yoshida, Tsuneyo Mimori, Sei Itsu Murota, Yasuo Ikeda

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)


P-selectin (GMP-140, PADGEM, CD62P) is a cell adhesion receptor which is believed to play an important role in inflammatory diseases by supporting leucocyte rolling. P-selectin is located on the granule membrane of Weibel- Palade bodies in resting endothelial cells and is expressed on the cell surface during cellular activation with various stimulators such as thrombin. Thereafter, P-selectin is internalized and sorted to the Golgi region and Weibel-Palade bodies again. However, whether P-selectin is re-expressed upon subsequent cellular stimulation has, to date, been unclear. To address this question, we measured the cellular content and surface expression of P- selectin, using indirect immunofluorescence and confocal laser cytometry. Surface expression of P-selectin reached a maximum < 2 min after thrombin stimulation and declined to basal levels after 18O min. Rechallenge with thrombin induced rapid surface re-expression of P-selectin, which was independent of de novo protein synthesis, since cycloheximide did not inhibit re-expression. Moreover, re-expressed P-selectin supported the adherence of HL60 promyelocytic cells. These results clearly demonstrated that functional P-selectin molecule was recycled after repeated stimulation with thrombin, raising the possibility that P-selectin is involved in chronic inflammation.

Original languageEnglish
Pages (from-to)348-355
Number of pages8
JournalBritish Journal of Haematology
Issue number2
Publication statusPublished - 1997


  • adhesion
  • chronic inflammation
  • degranulation
  • protein synthesis
  • recycling

ASJC Scopus subject areas

  • Hematology


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