Reaction monitoring of tocopherols with active nitrogen oxides by ultra high-speed liquid chromatography

Yoshiko Itoh, Tadashi Nishio, Hideko Kanazawa

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Ultra high-speed liquid chromatography (LC) has become increasingly popular in analytical research fields. This analytical system provides fast and efficient chromatographic separation over a wide range of flow rate and pressure. In this study, we applied an ultra high-speed LC system to monitor the reaction of α-, γ-, and δ-tocopherols with active nitrogen species. By using an ultra high-speed LC system equipped with a photo-diode array detector, short time analysis and detection of a wide range of reaction products were accomplished efficiently. The analysis time of tocopherol and its major oxidation products were greatly shortened compared to conventional HPLC methods (more than 10 times). The ultra reversed-phase LC was demonstrated to be as a powerful tool for monitoring rapid oxidation reactions of tocopherols with active nitrogen species.

Original languageEnglish
Pages (from-to)241-246
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume55
Issue number2
DOIs
Publication statusPublished - 2011 May 15

Fingerprint

Nitrogen Oxides
Tocopherols
Liquid chromatography
High Pressure Liquid Chromatography
Monitoring
Nitrogen
Oxidation
Reverse-Phase Chromatography
Reaction products
Diodes
Flow rate
Detectors
Pressure
Research

Keywords

  • Active nitrogen oxide
  • Reaction monitoring
  • Tocopherol
  • Ultra high-speed liquid chromatography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Drug Discovery
  • Pharmaceutical Science
  • Spectroscopy
  • Clinical Biochemistry

Cite this

@article{ab05376fb34140c098302c5bfb85022f,
title = "Reaction monitoring of tocopherols with active nitrogen oxides by ultra high-speed liquid chromatography",
abstract = "Ultra high-speed liquid chromatography (LC) has become increasingly popular in analytical research fields. This analytical system provides fast and efficient chromatographic separation over a wide range of flow rate and pressure. In this study, we applied an ultra high-speed LC system to monitor the reaction of α-, γ-, and δ-tocopherols with active nitrogen species. By using an ultra high-speed LC system equipped with a photo-diode array detector, short time analysis and detection of a wide range of reaction products were accomplished efficiently. The analysis time of tocopherol and its major oxidation products were greatly shortened compared to conventional HPLC methods (more than 10 times). The ultra reversed-phase LC was demonstrated to be as a powerful tool for monitoring rapid oxidation reactions of tocopherols with active nitrogen species.",
keywords = "Active nitrogen oxide, Reaction monitoring, Tocopherol, Ultra high-speed liquid chromatography",
author = "Yoshiko Itoh and Tadashi Nishio and Hideko Kanazawa",
year = "2011",
month = "5",
day = "15",
doi = "10.1016/j.jpba.2010.12.036",
language = "English",
volume = "55",
pages = "241--246",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
issn = "0731-7085",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Reaction monitoring of tocopherols with active nitrogen oxides by ultra high-speed liquid chromatography

AU - Itoh, Yoshiko

AU - Nishio, Tadashi

AU - Kanazawa, Hideko

PY - 2011/5/15

Y1 - 2011/5/15

N2 - Ultra high-speed liquid chromatography (LC) has become increasingly popular in analytical research fields. This analytical system provides fast and efficient chromatographic separation over a wide range of flow rate and pressure. In this study, we applied an ultra high-speed LC system to monitor the reaction of α-, γ-, and δ-tocopherols with active nitrogen species. By using an ultra high-speed LC system equipped with a photo-diode array detector, short time analysis and detection of a wide range of reaction products were accomplished efficiently. The analysis time of tocopherol and its major oxidation products were greatly shortened compared to conventional HPLC methods (more than 10 times). The ultra reversed-phase LC was demonstrated to be as a powerful tool for monitoring rapid oxidation reactions of tocopherols with active nitrogen species.

AB - Ultra high-speed liquid chromatography (LC) has become increasingly popular in analytical research fields. This analytical system provides fast and efficient chromatographic separation over a wide range of flow rate and pressure. In this study, we applied an ultra high-speed LC system to monitor the reaction of α-, γ-, and δ-tocopherols with active nitrogen species. By using an ultra high-speed LC system equipped with a photo-diode array detector, short time analysis and detection of a wide range of reaction products were accomplished efficiently. The analysis time of tocopherol and its major oxidation products were greatly shortened compared to conventional HPLC methods (more than 10 times). The ultra reversed-phase LC was demonstrated to be as a powerful tool for monitoring rapid oxidation reactions of tocopherols with active nitrogen species.

KW - Active nitrogen oxide

KW - Reaction monitoring

KW - Tocopherol

KW - Ultra high-speed liquid chromatography

UR - http://www.scopus.com/inward/record.url?scp=79952253007&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952253007&partnerID=8YFLogxK

U2 - 10.1016/j.jpba.2010.12.036

DO - 10.1016/j.jpba.2010.12.036

M3 - Article

C2 - 21330089

AN - SCOPUS:79952253007

VL - 55

SP - 241

EP - 246

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

SN - 0731-7085

IS - 2

ER -