Reaction monitoring of tocopherols with active nitrogen oxides by ultra high-speed liquid chromatography

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Ultra high-speed liquid chromatography (LC) has become increasingly popular in analytical research fields. This analytical system provides fast and efficient chromatographic separation over a wide range of flow rate and pressure. In this study, we applied an ultra high-speed LC system to monitor the reaction of α-, γ-, and δ-tocopherols with active nitrogen species. By using an ultra high-speed LC system equipped with a photo-diode array detector, short time analysis and detection of a wide range of reaction products were accomplished efficiently. The analysis time of tocopherol and its major oxidation products were greatly shortened compared to conventional HPLC methods (more than 10 times). The ultra reversed-phase LC was demonstrated to be as a powerful tool for monitoring rapid oxidation reactions of tocopherols with active nitrogen species.

Original languageEnglish
Pages (from-to)241-246
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume55
Issue number2
DOIs
Publication statusPublished - 2011 May 15

Keywords

  • Active nitrogen oxide
  • Reaction monitoring
  • Tocopherol
  • Ultra high-speed liquid chromatography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

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