Reactions of hypochlorite with catalase

Tadahiko Mashino, Irwin Fridovich

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

OCl-/HOCl imposed a rapid inactivation of catalase (hydrogen-peroxide: hydrogen-peroxide oxidoreductase, EC 1.11.1.6), some of which was slowly reversible upon subsequent exposure to H2O2. Ethanol accelerated this restoration of activity by H2O2. OCl- caused biphasic changes in the visible absorption spectrum of catalase, which were partially reversed by dithionite. A scheme of reactions involving axial ligation of one or two OCl- to heme iron, followed by heterolytic or homolytic cleavages of the OCl bond, is proposed to account for the behavior of the system.

Original languageEnglish
Pages (from-to)63-69
Number of pages7
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume956
Issue number1
DOIs
Publication statusPublished - 1988 Aug 31
Externally publishedYes

Fingerprint

Hypochlorous Acid
Catalase
Hydrogen Peroxide
Dithionite
Heme
Restoration
Ligation
Absorption spectra
Oxidoreductases
Ethanol
Iron

Keywords

  • Catalase
  • Hypochlorous acid
  • Sodium hypochlorite

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Structural Biology

Cite this

Reactions of hypochlorite with catalase. / Mashino, Tadahiko; Fridovich, Irwin.

In: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, Vol. 956, No. 1, 31.08.1988, p. 63-69.

Research output: Contribution to journalArticle

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