Reactive bone marrow stromal cells attenuate systemic inflammation via sTNFR1

Hiroshi Yagi, Alejandro Soto-Gutierrez, Nalu Navarro-Alvarez, Yaakov Nahmias, Yoni Goldwasser, Yuukou Kitagawa, Arno W. Tilles, Ronald G. Tompkins, Biju Parekkadan, Martin L. Yarmush

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

Excessive systemic inflammation following trauma, sepsis, or burn could lead to distant organ damage. The transplantation of bone marrow stromal cells or mesenchymal stem cells (MSCs) has been reported to be an effective treatment for several immune disorders by modulating the inflammatory response to injury. We hypothesized that MSCs can dynamically secrete systemic factors that can neutralize the activity of inflammatory cytokines. In this study, we showed that cocultured MSCs are able to decrease nuclear factor -B (NFB) activation in target epithelial cells incubated in inflammatory serum conditions. Proteomic screening revealed a responsive secretion of soluble tumor necrosis factor (TNF) receptor 1 (sTNFR1) when MSCs were exposed to lipopolysaccharide (LPS)-stimulated rat serum. The responsive effect was eliminated when NFB activation was blocked in MSCs. Intramuscular transplantation of MSCs in LPS-endotoxic rats decreased a panel of inflammatory cytokines and inflammatory infiltration of macrophages and neutrophils in lung, kidney, and liver when compared to controls. These results suggest that improvements of inflammatory responses in animal models after local transplantation of MSCs are at least, in part, explained by the NFB-dependent secretion of sTNFR1 by MSCs.

Original languageEnglish
Pages (from-to)1857-1864
Number of pages8
JournalMolecular Therapy
Volume18
Issue number10
DOIs
Publication statusPublished - 2010 Oct

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Tumor Necrosis Factor Receptors
Mesenchymal Stromal Cells
Inflammation
Mesenchymal Stem Cell Transplantation
Lipopolysaccharides
Cytokines
Neutrophil Infiltration
Immune System Diseases
Wounds and Injuries
Serum
Proteomics
Sepsis
Animal Models
Transplantation
Epithelial Cells
Macrophages
Kidney
Lung
Liver

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine
  • Genetics
  • Drug Discovery
  • Pharmacology

Cite this

Yagi, H., Soto-Gutierrez, A., Navarro-Alvarez, N., Nahmias, Y., Goldwasser, Y., Kitagawa, Y., ... Yarmush, M. L. (2010). Reactive bone marrow stromal cells attenuate systemic inflammation via sTNFR1. Molecular Therapy, 18(10), 1857-1864. https://doi.org/10.1038/mt.2010.155

Reactive bone marrow stromal cells attenuate systemic inflammation via sTNFR1. / Yagi, Hiroshi; Soto-Gutierrez, Alejandro; Navarro-Alvarez, Nalu; Nahmias, Yaakov; Goldwasser, Yoni; Kitagawa, Yuukou; Tilles, Arno W.; Tompkins, Ronald G.; Parekkadan, Biju; Yarmush, Martin L.

In: Molecular Therapy, Vol. 18, No. 10, 10.2010, p. 1857-1864.

Research output: Contribution to journalArticle

Yagi, H, Soto-Gutierrez, A, Navarro-Alvarez, N, Nahmias, Y, Goldwasser, Y, Kitagawa, Y, Tilles, AW, Tompkins, RG, Parekkadan, B & Yarmush, ML 2010, 'Reactive bone marrow stromal cells attenuate systemic inflammation via sTNFR1', Molecular Therapy, vol. 18, no. 10, pp. 1857-1864. https://doi.org/10.1038/mt.2010.155
Yagi H, Soto-Gutierrez A, Navarro-Alvarez N, Nahmias Y, Goldwasser Y, Kitagawa Y et al. Reactive bone marrow stromal cells attenuate systemic inflammation via sTNFR1. Molecular Therapy. 2010 Oct;18(10):1857-1864. https://doi.org/10.1038/mt.2010.155
Yagi, Hiroshi ; Soto-Gutierrez, Alejandro ; Navarro-Alvarez, Nalu ; Nahmias, Yaakov ; Goldwasser, Yoni ; Kitagawa, Yuukou ; Tilles, Arno W. ; Tompkins, Ronald G. ; Parekkadan, Biju ; Yarmush, Martin L. / Reactive bone marrow stromal cells attenuate systemic inflammation via sTNFR1. In: Molecular Therapy. 2010 ; Vol. 18, No. 10. pp. 1857-1864.
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