Reactive oxygen species mediate leukocyte-endothelium interactions in prostaglandin F-induced luteolysis in rats

Kazuhiro Minegishi, Mamoru Tanaka, Osamu Nishimura, Shinji Tanigaki, Kei Miyakoshi, Hitoshi Ishimoto, Yasunori Yoshimura

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We investigated the contribution of neutrophils to prostaglandin (PG)F-induced luteolysis and the role of reactive oxygen species (ROS) as potential mediators of neutrophil accumulation in regressing corpora lutea of superovulated rats. On day 8 of pseudopregnancy, subcutaneous injection of PGF (500 μg) significantly increased rhodamine 6G-labeled leukocyte adhesion in luteal venules, as observed by intravital microscopy. Neutrophil accumulation was confirmed by significantly increased myeloperoxidase (MPO) activity. Pretreatment with anti-leukocyte antibody (CD18-directed monoclonal antibody, WT-3) significantly inhibited the PGF-induced increases in adherent leukocytes and MPO activity. Anti-leukocyte antibody also maintained serum progesterone concentrations. Pretreatment with oxygen free radical scavengers, superoxide dismutase (50,000 U/kg) and catalase (90,000 U/kg), also attenuated these PGF-induced alterations. Corpora lutea preloaded with dichlorodihydrofluorescein diacetate succinimidyl ester, a fluorescent indicator for determining intracellular ROS generation, exhibited an increase in fluorescence after PGF treatment. These findings suggest that leukocyte-endothelium interactions mediated by ROS generation are important in PGF-induced luteolysis in rats.

Original languageEnglish
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume283
Issue number6 46-6
Publication statusPublished - 2002 Dec 1

Fingerprint

Luteolysis
Dinoprost
Endothelium
Rats
Reactive Oxygen Species
Leukocytes
Corpus Luteum
Neutrophils
Peroxidase
Anti-Idiotypic Antibodies
Pseudopregnancy
Free Radical Scavengers
Venules
Antibodies
Subcutaneous Injections
Catalase
Superoxide Dismutase
Progesterone
Esters
Adhesion

Keywords

  • CD18 integrin
  • Corpus luteum
  • Intravital microscopy
  • Myeloperoxidase activity
  • Neutrophil

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry

Cite this

Reactive oxygen species mediate leukocyte-endothelium interactions in prostaglandin F-induced luteolysis in rats. / Minegishi, Kazuhiro; Tanaka, Mamoru; Nishimura, Osamu; Tanigaki, Shinji; Miyakoshi, Kei; Ishimoto, Hitoshi; Yoshimura, Yasunori.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 283, No. 6 46-6, 01.12.2002.

Research output: Contribution to journalArticle

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